A0A926S8B7 · A0A926S8B7_9HYPH

  • Protein
    Ribonuclease E
  • Gene
    rne
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site434Mg2+ (UniProtKB | ChEBI); catalytic
Binding site477Mg2+ (UniProtKB | ChEBI); catalytic
Binding site535Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site538Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • ORF names
      HK439_23875

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • PHM038
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Stappiaceae > Roseibium

Accessions

  • Primary accession
    A0A926S8B7

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Homotetramer formed by a dimer of dimers.

Family & Domains

Features

Showing features for domain, compositional bias, region.

TypeIDPosition(s)Description
Domain38-250S1 motif
Compositional bias93-113Basic and acidic residues
Region93-219Disordered
Compositional bias168-203Acidic residues
Compositional bias204-219Basic and acidic residues
Region535-538Required for zinc-mediated homotetramerization and catalytic activity
Region620-862Disordered
Compositional bias639-654Acidic residues
Compositional bias675-694Basic and acidic residues
Compositional bias701-725Basic and acidic residues
Compositional bias837-854Basic and acidic residues

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    862
  • Mass (Da)
    95,927
  • Last updated
    2023-02-22 v1
  • Checksum
    AE19801DF4343A9E
MANKMLIDAAHPEETRVVVVRGNRVEEFDFEAANRKQLRGNIYLAKVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQIPVADRQALLDAEHHAEQKDDSGEEDEAPARKPRRGRRSKAAASKTDETVASEPVDSDDREEKSEDAAEADQADAGVEAATSEDVTEDRSAETEDETEARASDEDDDGEADGDDQVESVGAEDALEELPERRPPPRRQYKIQEVIKRRQVILVQVVKEERGNKGAALTTYLSLAGRYSVLMPNTGRGGGISRKITQPTDRKRLKKIASELDVPEGMGVILRTAGASRTKAEIKRDFEYLMRLWENVRELTLKSSAPSLVYEEGSLIKRSIRDLYNKDINEVLVAGDEGYREAKDFMRMLMPSHAKNVQPYRDPSPIFIRNGVEPQLDAMFSPQVTLKSGGYIVINQTEALVSIDVNSGKSTREHNIEDTALQTNLEAAEEVTRQLRLRDLAGLVVIDFIDMEENKNNRTVERRLKDCLKNDRARIQVGRISHFGLLEMSRQRIRTGVLESSTTPCPHCSGTGMIRSVESVALHVLRSLEDNLLKGATHNLVIRTTTPVALYILNQKRANLSDLEARFGVWIEVQADDMVNGQHYVLERADPVDRDRQPQVVPIVQPDTIIPEEEEEEDLTETPERSEDEGDRDRRRKRRRKRRRGGADGSSEGAEARAPREEDGEGGETGDEDADRAGGQEGEGGSSDDEPRRKRRRGRRGGRRGRRNGENGDETLEVEAGTEAETGTGAETPEDGEAQEETETREVPSEPEAAPVEAVAEAEAEAEAEVSEAAEEPAAPAEEEKGETLVKTPAQATEPAEPVVTSEKASDSEDDADKKPKRGGWWQRRSFF

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias93-113Basic and acidic residues
Compositional bias168-203Acidic residues
Compositional bias204-219Basic and acidic residues
Compositional bias639-654Acidic residues
Compositional bias675-694Basic and acidic residues
Compositional bias701-725Basic and acidic residues
Compositional bias837-854Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JABFCZ010000034
EMBL· GenBank· DDBJ
MBD1549310.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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