A0A926NW58 · A0A926NW58_9HYPH
- ProteinCarbamoyltransferase HypF
- GenehypF
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids773 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in the maturation of [NiFe] hydrogenases. Along with HypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases. HypF functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carboxamido moiety in an ATP-dependent reaction to the thiolate of the C-terminal cysteine of HypE yielding a protein-S-carboxamide.
Catalytic activity
- ATP + C-terminal L-cysteinyl-[HypE protein] + carbamoyl phosphate + H2O = AMP + C-terminal S-carboxamide-L-cysteinyl-[HypE protein] + diphosphate + H+ + phosphate
Pathway
Protein modification; [NiFe] hydrogenase maturation.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 25 | |||||
Sequence: R | ||||||
Active site | 43 | |||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | carboxyl- or carbamoyltransferase activity | |
Molecular Function | double-stranded RNA binding | |
Molecular Function | hydrolase activity | |
Molecular Function | ligase activity | |
Molecular Function | zinc ion binding | |
Biological Process | protein maturation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCarbamoyltransferase HypF
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Stappiaceae > Roseibium
Accessions
- Primary accessionA0A926NW58
Proteomes
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 10-97 | Acylphosphatase-like | ||||
Sequence: GRRIRIKGLVQGVGFRPHVWRLATDHGISGSVRNDGEGVEIDAWAEADRLDRFLAAIRSEAPPLARIDSISFKDLSEPSPGTAFEIVK | ||||||
Domain | 205-389 | YrdC-like | ||||
Sequence: ADPLQETARRIGQEQIAAIKGIGGFHLACDALNETAVTELRRRKRRPVKPLALMAASLSEIRKYCRVTPAEEAQLKSAAAPIVLLEVQGEPLAPFIAPGQDRLGFMLPYTPLHHHLLAAVDGPLVFTSGNLSDEPQAIDNDDARGRLSEIADVWLMHDREIVNRLDDSVVRIDAPGPQILRRARG |
Sequence similarities
Belongs to the carbamoyltransferase HypF family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length773
- Mass (Da)83,162
- Last updated2023-02-22 v1
- Checksum8196A087BEE9A822