A0A921NCW7 · A0A921NCW7_9BACL
- ProteintRNA-specific adenosine deaminase
- GenetadA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids483 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2).
Nitronate monooxygenase that uses molecular oxygen to catalyze the oxidative denitrification of alkyl nitronates. Acts on propionate 3-nitronate (P3N), the presumed physiological substrate. Probably functions in the detoxification of P3N, a metabolic poison produced by plants and fungi as a defense mechanism.
Catalytic activity
- 3 propionate 3-nitronate + 3 O2 + H2O = 3 3-oxopropanoate + 2 nitrate + nitrite + H2O2 + 3 H+
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 zinc ion per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 56 | Zn2+ (UniProtKB | ChEBI); catalytic | |||
Active site | 58 | Proton donor | |||
Binding site | 86 | Zn2+ (UniProtKB | ChEBI); catalytic | |||
Binding site | 89 | Zn2+ (UniProtKB | ChEBI); catalytic | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | nitronate monooxygenase activity | |
Molecular Function | tRNA-specific adenosine-34 deaminase activity | |
Molecular Function | zinc ion binding | |
Biological Process | response to toxic substance | |
Biological Process | tRNA wobble adenosine to inosine editing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-specific adenosine deaminase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Planococcaceae > Metalysinibacillus
Accessions
- Primary accessionA0A921NCW7
Proteomes
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 5-114 | CMP/dCMP-type deaminase | |||
Sequence similarities
Belongs to the cytidine and deoxycytidylate deaminase family. ADAT2 subfamily.
Belongs to the nitronate monooxygenase family. NMO class I subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length483
- Mass (Da)53,023
- Last updated2023-02-22 v1
- MD5 ChecksumD4FCE41CB08DBDE1F25555A972EC1A23
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DYTV01000074 EMBL· GenBank· DDBJ | HJH11218.1 EMBL· GenBank· DDBJ | Genomic DNA |