A0A919LQ24 · A0A919LQ24_KLEPN
- ProteinRibonuclease T
- Genernt
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids215 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Trims short 3' overhangs of a variety of RNA species, leaving a one or two nucleotide 3' overhang. Responsible for the end-turnover of tRNA: specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis.
Cofactor
Note: Binds two Mg2+ per subunit. The active form of the enzyme binds two Mg2+ ions in its active site. The first Mg2+ forms only one salt bridge with the protein.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 23 | Mg2+ 1 (UniProtKB | ChEBI); catalytic | |||
Binding site | 23 | Mg2+ 2 (UniProtKB | ChEBI); catalytic | |||
Binding site | 25 | Mg2+ 2 (UniProtKB | ChEBI); catalytic | |||
Site | 29 | Important for substrate binding and specificity | |||
Site | 77 | Important for substrate binding and specificity | |||
Site | 124 | Important for substrate binding and specificity | |||
Site | 146 | Important for substrate binding and specificity | |||
Active site | 181 | Proton donor/acceptor | |||
Binding site | 181 | Mg2+ 2 (UniProtKB | ChEBI); catalytic | |||
Binding site | 186 | Mg2+ 2 (UniProtKB | ChEBI); catalytic | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 3'-5' exonuclease activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | nucleic acid binding | |
Molecular Function | RNA exonuclease activity, producing 5'-phosphomonoesters | |
Biological Process | DNA replication proofreading | |
Biological Process | tRNA processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibonuclease T
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Klebsiella/Raoultella group > Klebsiella
Accessions
- Primary accessionA0A919LQ24
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length215
- Mass (Da)23,590
- Last updated2023-02-22 v1
- Checksum00A34080782232B9
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BNFF01000001 EMBL· GenBank· DDBJ | GHK51148.1 EMBL· GenBank· DDBJ | Genomic DNA |