A0A919LQ24 · A0A919LQ24_KLEPN

Function

function

Trims short 3' overhangs of a variety of RNA species, leaving a one or two nucleotide 3' overhang. Responsible for the end-turnover of tRNA: specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds two Mg2+ per subunit. The active form of the enzyme binds two Mg2+ ions in its active site. The first Mg2+ forms only one salt bridge with the protein.

Features

Showing features for binding site, site, active site.

121520406080100120140160180200
Type
IDPosition(s)Description
Binding site23Mg2+ 1 (UniProtKB | ChEBI); catalytic
Binding site23Mg2+ 2 (UniProtKB | ChEBI); catalytic
Binding site25Mg2+ 2 (UniProtKB | ChEBI); catalytic
Site29Important for substrate binding and specificity
Site77Important for substrate binding and specificity
Site124Important for substrate binding and specificity
Site146Important for substrate binding and specificity
Active site181Proton donor/acceptor
Binding site181Mg2+ 2 (UniProtKB | ChEBI); catalytic
Binding site186Mg2+ 2 (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function3'-5' exonuclease activity
Molecular Functionmagnesium ion binding
Molecular Functionnucleic acid binding
Molecular FunctionRNA exonuclease activity, producing 5'-phosphomonoesters
Biological ProcessDNA replication proofreading
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease T
  • EC number
  • Alternative names
    • Exoribonuclease T
      (RNase T
      )

Gene names

    • Name
      rnt
    • ORF names
      KPZU09_08840

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Zam_UTH_09
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Klebsiella/Raoultella group > Klebsiella

Accessions

  • Primary accession
    A0A919LQ24

Proteomes

Subcellular Location

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain18-203Exonuclease

Sequence similarities

Belongs to the RNase T family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    215
  • Mass (Da)
    23,590
  • Last updated
    2023-02-22 v1
  • Checksum
    00A34080782232B9
MSENAQLNGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEHGWLMPDETLHFHVEPFEGANLQPEALAFNGINPHDPQRGAVSEYDALHAIFKMVRKGMKESDCSRAIMVAHNATFDHSFTMTAAERAGLKRNPFHPFVTFDTAALSGLALGQTVLSKACIAAGMPFDGAQAHSALYDTEQTAQLFCEIVNRWKRLGGWPPPVATPE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BNFF01000001
EMBL· GenBank· DDBJ
GHK51148.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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