A0A915AGN3 · A0A915AGN3_PARUN

Function

function

Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Features

Showing features for binding site.

165750100150200250300350400450500550600650
TypeIDPosition(s)Description
Binding site288ATP (UniProtKB | ChEBI)
Binding site337-344ATP (UniProtKB | ChEBI)
Binding site402-403ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular ComponentP-body
Cellular ComponentPAN complex
Molecular FunctionATP binding
Molecular Functionpoly(A) binding
Molecular Functionprotein kinase activity
Biological ProcessmRNA processing
Biological Processnuclear-transcribed mRNA poly(A) tail shortening
Biological Processpositive regulation of cytoplasmic mRNA processing body assembly
Biological Processprotein phosphorylation

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    PAN2-PAN3 deadenylation complex subunit PAN3
  • Alternative names
    • PAB1P-dependent poly(A)-specific ribonuclease
    • Poly(A)-nuclease deadenylation complex subunit 3
      (PAN deadenylation complex subunit 3
      )

Gene names

    • Name
      PAN3

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Spirurina > Ascaridomorpha > Ascaridoidea > Ascarididae > Parascaris

Accessions

  • Primary accession
    A0A915AGN3

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer. Forms a heterotrimer with a catalytic subunit PAN2 to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts (via PAM-2 motif) with poly(A)-binding protein (via PABC domain), conferring substrate specificity of the enzyme complex.

Family & Domains

Features

Showing features for region, domain, coiled coil.

Type
IDPosition(s)Description
Region33-71Disordered
Domain252-546Protein kinase
Coiled coil506-544
Region545-657Knob domain

Domain

Contains a pseudokinase domain. The protein kinase domain is predicted to be catalytically inactive because some of the residues important for catalytic activity are substituted and it lacks the equivalent of the binding site for a peptide substrate. However, it has retained an ATP-binding site and ATP-binding is required for mRNA degradation, stimulating the activity of the PAN2 nuclease in vitro. The nucleotide-binding site is juxtaposed to the RNase active site of PAN2 in the complex and may actually bind nucleosides of a poly(A) RNA rather than ATP, feeding the poly(A)-tail to the active site of the deadenylase and thus increasing the efficiency with which this distributive enzyme degrades oligo(A) RNAs.
The N-terminal zinc finger binds to poly(A) RNA.
The pseudokinase domain, the coiled-coil (CC), and C-terminal knob domain (CK) form a structural unit (PKC) that forms an extensive high-affinity interaction surface for PAN2.

Sequence similarities

Belongs to the protein kinase superfamily. PAN3 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    657
  • Mass (Da)
    72,064
  • Last updated
    2023-02-22 v1
  • Checksum
    E76BD9C04C370CAF
GNSAASDIFAACDHPATSVGAVPAGSRLAQYLHGSNAQPPVPRQSSWNSPLSQSQSSGTPYGISSQSTLQPSNVPSYMASFAQMGLGDDKDLNPSISVAPFSMPSSSQRPINYGPTTQVLTASPVLHDPLRASGHLAEGGSASGYMQENYGGTTYFFQQGANAGLEQPREDQPNDGRIVVTTPGCFAYQGPLPHIGKFKPKGSTAQSFFTSPELKMELLQRQLAVQCRADPTVYGDLPQALEHFQNLVPLEPLKMPSLGHGERGVLGPYTSSVYKATSIRDGMPYCIRRIHNYRVNSAKHLSLADNWKKLIHANVVQLREVLVTRHFGDHSLLFVYDFHPLSETMKFRHLSSRINGMSTNFMDGMSGGGLQESLIWTYIVQLSSALRAIHSSGMAARTVDPTKIIVFSKTKLMLSSCGVLDVINPENNHNSIQHQQEDLCGLGRVIVALAMGSLHAARRENLSASLAVISQHYTADMKNIISLLLSASAQGSRVRSINEVMPMIGARFYAQLELAQMKNDLLENELSKELENGRLFRLLCKLNTIIERPQFQMDMSWSETGDRYLLKLFRDYLFHQVTEAGKPWIDMAHIISCLNKLDAGLNEKIQLVSRDGDNVLVVSFLDLRRCLENAFRELHAAATAPPPPVSIAPPLLPMAPR

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A915AFQ2A0A915AFQ2_PARUNPAN3674

Keywords

Genome annotation databases

Similar Proteins

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