A0A8X8RMR1 · A0A8X8RMR1_9CAUD
- ProteinPre-baseplate central spike protein Gp5
- Gene5
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids576 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Baseplate central spike protein Gp5*
Baseplate central spike complex-associated lysozyme that is essential for the localized hydrolysis of bacterial cell wall, so that the tail tube, through which the phage DNA is ejected, can penetrate to the host inner membrane. The tail lysozyme complex at the tip of the tail tube penetrates through the outer membrane into the periplasm and during that process, gp5* dissociates from gp5C and activated. Due to the lower pH in the periplasm, gp5* would dissociate from gp27 which probably still binds to the tip of the tube. This way, lysozyme domain is released and locally digests the peptidoglycan layer to make a hole to let the tube penetrate to the inner membrane. Involved in the tail assembly.
Catalytic activity
Features
Showing features for active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 184 | Proton donor | ||||
Sequence: E | ||||||
Active site | 193 | Nucleophile | ||||
Sequence: D | ||||||
Site | 351-352 | Cleavage | ||||
Sequence: SA |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | virus tail, baseplate | |
Molecular Function | lysozyme activity | |
Biological Process | cell wall macromolecule catabolic process | |
Biological Process | defense response to bacterium | |
Biological Process | killing of cells of another organism | |
Biological Process | peptidoglycan catabolic process | |
Biological Process | symbiont entry into host cell via disruption of host cell envelope | |
Biological Process | symbiont entry into host cell via disruption of host cell wall peptidoglycan | |
Biological Process | viral tail assembly |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended namePre-baseplate central spike protein Gp5
- Short namesPre-Gp5
- Alternative names
- Cleaved into 2 chains
Gene names
Organism names
- Organism
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Straboviridae > Tevenvirinae > Tequatrovirus
Accessions
- Primary accessionA0A8X8RMR1
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_5036521264 | 1-351 | Baseplate central spike protein Gp5* | |||
Sequence: MEMISNNLNWFVGVVEDRMDPLKLGRVRVRVVGLHPPQRAQGDVMGIPTEKLPWMSVIQPITSAAMSGIGGSVTGPVEGTRVYGHFLDKWKTNGIVLGTYGGIVREKPNRLEGFSDPTGQYPRRLGNDTNVLNQGGEVGYDSSSNIIQDSNLDTAINPDDRPLSEIPTDDNPNMSMAEMLRRDEGLRLKVYWDTEGYPTIGIGHLIMKQPVRDMAQINKVLSKQVGREITGNPGSITMEEATTLFERDLADMQRDIKSHSKVGPVWQAVNRSRQMALENMAFQMGVGGVAKFNTMLTAMLAGDWEKAYKAGRDSLWYQQTKGRASRVTMIILTGNLESYGVEVKTPSRSLS | ||||||
Chain | PRO_5036521263 | 1-576 | Pre-baseplate central spike protein Gp5 | |||
Sequence: MEMISNNLNWFVGVVEDRMDPLKLGRVRVRVVGLHPPQRAQGDVMGIPTEKLPWMSVIQPITSAAMSGIGGSVTGPVEGTRVYGHFLDKWKTNGIVLGTYGGIVREKPNRLEGFSDPTGQYPRRLGNDTNVLNQGGEVGYDSSSNIIQDSNLDTAINPDDRPLSEIPTDDNPNMSMAEMLRRDEGLRLKVYWDTEGYPTIGIGHLIMKQPVRDMAQINKVLSKQVGREITGNPGSITMEEATTLFERDLADMQRDIKSHSKVGPVWQAVNRSRQMALENMAFQMGVGGVAKFNTMLTAMLAGDWEKAYKAGRDSLWYQQTKGRASRVTMIILTGNLESYGVEVKTPSRSLSAMAATVAKSSDPADPPIPNDSRILFKEPVSSYKGEYPYVHTMETESGHIQEFDDTPGQERYRLVHPTGTYEEVSPSGRRTRKTVDNLYDITNADGNFLVAGDKKTNVGGSEIYYNMDNRLHQIDGSNTIFVRGDETKTVEGNGTILVKGNVTIVVEGNADITVKGDATTLVEGNQTNTVNGNLSWKVAGTVDWDVGGDWTEKMASMSSISSGQYTIDGSRIDVGL | ||||||
Chain | PRO_5036521262 | 352-576 | Gp5C | |||
Sequence: AMAATVAKSSDPADPPIPNDSRILFKEPVSSYKGEYPYVHTMETESGHIQEFDDTPGQERYRLVHPTGTYEEVSPSGRRTRKTVDNLYDITNADGNFLVAGDKKTNVGGSEIYYNMDNRLHQIDGSNTIFVRGDETKTVEGNGTILVKGNVTIVVEGNADITVKGDATTLVEGNQTNTVNGNLSWKVAGTVDWDVGGDWTEKMASMSSISSGQYTIDGSRIDVGL |
Post-translational modification
Pre-baseplate central spike protein Gp5
In the fully assembled virus, gp5 precursor is cleaved to form the mature tail lysozyme gp5*, and a C-terminus fragment, gp5C. The two fragments remain associated with the virion. The enzymatic activity of the precursor is about 10% of that of mature gp5*.
Expression
Keywords
- Developmental stage
Interaction
Subunit
Baseplate central spike protein Gp5*
Monomer. The central spike complex, which creates an extension of the tail tube, is made up of three copies of the gp27-gp5*-gp5C complex and one copy of gp5.4. Part of the baseplate macromolecular complex which consists of gp5*, gp5C, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube).
Gp5C
Homotrimer. The central spike complex, which creates an extension of the tail tube, is made up of three copies of the gp27-gp5*-gp5C complex and one copy of gp5.4. Part of the baseplate macromolecular complex which consists of gp5*, gp5C, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube).
Pre-baseplate central spike protein Gp5
Homotrimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 33-173 | Protein Gp5 N-terminal OB-fold | ||||
Sequence: GLHPPQRAQGDVMGIPTEKLPWMSVIQPITSAAMSGIGGSVTGPVEGTRVYGHFLDKWKTNGIVLGTYGGIVREKPNRLEGFSDPTGQYPRRLGNDTNVLNQGGEVGYDSSSNIIQDSNLDTAINPDDRPLSEIPTDDNPN |
Sequence similarities
Belongs to the glycosyl hydrolase 24 family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length576
- Mass (Da)63,231
- Last updated2022-12-14 v1
- Checksum2AC5726D9B0947BB