A0A8X8RMC2 · A0A8X8RMC2_9CAUD
- ProteinPre-baseplate central spike protein Gp5
- Gene5
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids575 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Baseplate central spike protein Gp5*
Baseplate central spike complex-associated lysozyme that is essential for the localized hydrolysis of bacterial cell wall, so that the tail tube, through which the phage DNA is ejected, can penetrate to the host inner membrane. The tail lysozyme complex at the tip of the tail tube penetrates through the outer membrane into the periplasm and during that process, gp5* dissociates from gp5C and activated. Due to the lower pH in the periplasm, gp5* would dissociate from gp27 which probably still binds to the tip of the tube. This way, lysozyme domain is released and locally digests the peptidoglycan layer to make a hole to let the tube penetrate to the inner membrane. Involved in the tail assembly.
Catalytic activity
Features
Showing features for active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 184 | Proton donor | |||
Active site | 193 | Nucleophile | |||
Site | 351-352 | Cleavage | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | virus tail, baseplate | |
Molecular Function | lysozyme activity | |
Biological Process | cell wall macromolecule catabolic process | |
Biological Process | defense response to bacterium | |
Biological Process | killing of cells of another organism | |
Biological Process | peptidoglycan catabolic process | |
Biological Process | symbiont entry into host cell via disruption of host cell envelope | |
Biological Process | symbiont entry into host cell via disruption of host cell wall peptidoglycan | |
Biological Process | viral tail assembly |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended namePre-baseplate central spike protein Gp5
- Short namesPre-Gp5
- Alternative names
- Cleaved into 2 chains
Gene names
Organism names
- Organism
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Straboviridae > Tevenvirinae > Tequatrovirus
Accessions
- Primary accessionA0A8X8RMC2
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_5036521742 | 1-351 | Baseplate central spike protein Gp5* | ||
Chain | PRO_5036521743 | 1-575 | Pre-baseplate central spike protein Gp5 | ||
Chain | PRO_5036521744 | 352-575 | Gp5C | ||
Post-translational modification
Pre-baseplate central spike protein Gp5
In the fully assembled virus, gp5 precursor is cleaved to form the mature tail lysozyme gp5*, and a C-terminus fragment, gp5C. The two fragments remain associated with the virion. The enzymatic activity of the precursor is about 10% of that of mature gp5*.
Expression
Keywords
- Developmental stage
Interaction
Subunit
Baseplate central spike protein Gp5*
Monomer. The central spike complex, which creates an extension of the tail tube, is made up of three copies of the gp27-gp5*-gp5C complex and one copy of gp5.4. Part of the baseplate macromolecular complex which consists of gp5*, gp5C, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube).
Gp5C
Homotrimer. The central spike complex, which creates an extension of the tail tube, is made up of three copies of the gp27-gp5*-gp5C complex and one copy of gp5.4. Part of the baseplate macromolecular complex which consists of gp5*, gp5C, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube).
Pre-baseplate central spike protein Gp5
Homotrimer.
Structure
Sequence
- Sequence statusComplete
- Length575
- Mass (Da)63,132
- Last updated2022-12-14 v1
- ChecksumB560FD9B0947BB79
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
ON528739 EMBL· GenBank· DDBJ | URY14334.1 EMBL· GenBank· DDBJ | Genomic DNA |