A0A8X8RMC2 · A0A8X8RMC2_9CAUD

  • Protein
    Pre-baseplate central spike protein Gp5
  • Gene
    5
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Baseplate central spike protein Gp5*

Baseplate central spike complex-associated lysozyme that is essential for the localized hydrolysis of bacterial cell wall, so that the tail tube, through which the phage DNA is ejected, can penetrate to the host inner membrane. The tail lysozyme complex at the tip of the tail tube penetrates through the outer membrane into the periplasm and during that process, gp5* dissociates from gp5C and activated. Due to the lower pH in the periplasm, gp5* would dissociate from gp27 which probably still binds to the tip of the tube. This way, lysozyme domain is released and locally digests the peptidoglycan layer to make a hole to let the tube penetrate to the inner membrane. Involved in the tail assembly.

Catalytic activity

  • Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
    EC:3.2.1.17 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site, site.

157550100150200250300350400450500550
Type
IDPosition(s)Description
Active site184Proton donor
Active site193Nucleophile
Site351-352Cleavage

GO annotations

AspectTerm
Cellular Componentvirus tail, baseplate
Molecular Functionlysozyme activity
Biological Processcell wall macromolecule catabolic process
Biological Processdefense response to bacterium
Biological Processkilling of cells of another organism
Biological Processpeptidoglycan catabolic process
Biological Processsymbiont entry into host cell via disruption of host cell envelope
Biological Processsymbiont entry into host cell via disruption of host cell wall peptidoglycan
Biological Processviral tail assembly

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Pre-baseplate central spike protein Gp5
  • Short names
    Pre-Gp5
  • Alternative names
    • Peptidoglycan hydrolase gp5
      (EC:3.2.1.17
      ) . EC:3.2.1.17 (UniProtKB | ENZYME | Rhea)
  • Cleaved into 2 chains

Gene names

    • Name
      5

Organism names

  • Taxonomic identifier
  • Organism
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Straboviridae > Tevenvirinae > Tequatrovirus

Accessions

  • Primary accession
    A0A8X8RMC2

Proteomes

Subcellular Location

Baseplate central spike protein Gp5*

Virion
Note: Present in 3 copies in the baseplate.

Gp5C

Virion
Note: Present in 3 copies in the baseplate.

Pre-baseplate central spike protein Gp5

Virion
Note: Present in the baseplate.

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_50365217421-351Baseplate central spike protein Gp5*
ChainPRO_50365217431-575Pre-baseplate central spike protein Gp5
ChainPRO_5036521744352-575Gp5C

Post-translational modification

Pre-baseplate central spike protein Gp5

In the fully assembled virus, gp5 precursor is cleaved to form the mature tail lysozyme gp5*, and a C-terminus fragment, gp5C. The two fragments remain associated with the virion. The enzymatic activity of the precursor is about 10% of that of mature gp5*.

Expression

Keywords

Interaction

Subunit

Baseplate central spike protein Gp5*

Monomer. The central spike complex, which creates an extension of the tail tube, is made up of three copies of the gp27-gp5*-gp5C complex and one copy of gp5.4. Part of the baseplate macromolecular complex which consists of gp5*, gp5C, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube).

Gp5C

Homotrimer. The central spike complex, which creates an extension of the tail tube, is made up of three copies of the gp27-gp5*-gp5C complex and one copy of gp5.4. Part of the baseplate macromolecular complex which consists of gp5*, gp5C, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube).

Pre-baseplate central spike protein Gp5

Homotrimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain33-173Protein Gp5 N-terminal OB-fold

Sequence similarities

Belongs to the glycosyl hydrolase 24 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    575
  • Mass (Da)
    63,132
  • Last updated
    2022-12-14 v1
  • Checksum
    B560FD9B0947BB79
MEMISNNLNWFVGVVEDRMDPLKLGRVRVRVVGLHPPQRAQGDVMGIPTEKLPWMSVIQPITSAAMSGIGGSVTGPVEGTRVYGHFLDKWKTNGIVLGTYGGIVREKPNRLEGFSDPTGQYPRRLGNDTNVLNQGGEVGYDSSSNIIQDSNLDTAINPDDRPLSEIPTDDNPNMSMAEMLRRDEGLRLKVYWDTEGYPTIGIGHLIMKQPVRDMAQINKVLSKQVGREITGNPGSITMEEATTLFERDLADMQRDIKSHSKVGPVWQAVNRSRQMALENMAFQMGVGGVAKFNTMLTAMLAGDWEKAYKAGRDSLWYQQTKGRASRVTMIILTGNLESYGVEVKTPSRSLSAMAATVAKSSDPADPPIPNDSRILFKEPVSSYKGEYPYVHTMETESGHIQEFDDTPGQERYRLVHPTGTYEEVSPSGRRTRKTVDNLYDITNADGNFLVAGDKKTNVGGSEIYYNMDNRLHQIDGSNTIFVRGDETKTVEGNGTILVKGNVTIVVEGNADITVKGDATTLVEGNQTNTVNGNLSWKVAGTVDWDVGGDWTEKMASMSSISSGQYTIDGSRIDIG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
ON528739
EMBL· GenBank· DDBJ
URY14334.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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