A0A8X8M5L2 · A0A8X8M5L2_9CAUD

Function

function

Endolysin with amidase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and breaking down the peptidoglycan layer.
Plays an important role in the switch between viral transcription and genome replication. Once produced in sufficient amount, interacts with and inhibits the viral RNA polymerase that becomes unable to produce additional late transcripts. This lysozyme-polymerase complex in turn plays an active role in viral genome replication and packaging.

Catalytic activity

  • Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
    EC:3.5.1.28 (UniProtKB | ENZYME | Rhea)

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Zn2+ is required for amidase activity.

Activity regulation

Binding to the viral RNA polymerase inhibits amidase activity.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site18Zn2+ (UniProtKB | ChEBI)
Site47Essential for amidase activity and zinc hydrate coordination
Binding site123Zn2+ (UniProtKB | ChEBI)
Binding site131Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell cytoplasm
Molecular FunctionN-acetylmuramoyl-L-alanine amidase activity
Molecular Functionzinc ion binding
Biological Processdefense response to bacterium
Biological Processnegative regulation of viral transcription
Biological Processpeptidoglycan catabolic process
Biological Processviral release from host cell by cytolysis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Endolysin
  • EC number
  • Alternative names
    • N-acetylmuramoyl-L-alanine amidase

Organism names

  • Taxonomic identifier
  • Organism
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Autographiviridae > Studiervirinae > Teseptimavirus

Accessions

  • Primary accession
    A0A8X8M5L2

Proteomes

Subcellular Location

Host cytoplasm
Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.

Keywords

Expression

Keywords

Interaction

Subunit

Interacts with the viral RNA polymerase.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-124Peptidoglycan recognition protein family
Domain1-133N-acetylmuramoyl-L-alanine amidase

Sequence similarities

Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    151
  • Mass (Da)
    17,064
  • Last updated
    2022-12-14 v1
  • Checksum
    BBD42CC4010E5006
MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDVGYHFIIKRDGTVEEGRDEMAVGSHAKGHNHNSIGVCLVGGIDDKGKFDANFTPAQMQSLRSLLVTLLAKYEGAVLRAHHEVAPKACPSFNLKRWWEENELVTSDHE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
ON528719
EMBL· GenBank· DDBJ
URY10875.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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