A0A8X8M5D0 · A0A8X8M5D0_9CAUD
- ProteinPeptidoglycan transglycosylase gp16
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1322 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Component of the cylindrical core that assembles on the inner surface of the capsid during capsid formation and plays a role in viral DNA ejection into the host cell. The inner core is composed of stacked rings of gp14, gp15 and gp16 proteins. Following binding to the host cell surface, the internal core is disassembled and gp16 is ejected along with gp14 and gp15 into the infected cell. Gp16 probably inserts in the host inner membrane and remains associated with gp15. The gp15-gp16 complex binds to both the viral DNA and the host inner membrane, probably escorting the leading end of the genome through the periplasm and controlling the extent of DNA translocated into the host cell. Functions as an exolysin that catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine residues in peptidoglycans allowing the local digestion of the bacterial peptidoglycan wall.
Catalytic activity
- Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 36 | |||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell plasma membrane | |
Cellular Component | membrane | |
Cellular Component | virion component | |
Molecular Function | hydrolase activity | |
Molecular Function | lytic transglycosylase activity | |
Biological Process | defense response to bacterium | |
Biological Process | killing of cells of another organism | |
Biological Process | peptidoglycan metabolic process | |
Biological Process | symbiont entry into host cell via disruption of host cell envelope | |
Biological Process | symbiont entry into host cell via disruption of host cell wall peptidoglycan | |
Biological Process | symbiont genome ejection through host cell envelope, short tail mechanism |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePeptidoglycan transglycosylase gp16
- EC number
- Alternative names
Organism names
- Organism
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Autographiviridae > Studiervirinae > Teetrevirus
Accessions
- Primary accessionA0A8X8M5D0
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Host cell inner membrane ; Single-pass membrane protein
Note: The gp15-gp16 complex spans the periplasm and the cytoplasmic membrane.
Features
Showing features for topological domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-1137 | Periplasmic | ||||
Sequence: MSYDKTKPSEYDGLFQKAADEHGVSYDLLRKLAFNESGFNPKAKSPTGPLGLMQFTKGTAAGLGLKVTGGDDDERFNPELAVDAAARHLSDLVRKYDGDELKAALAYNQGEGRNGAPQMQAYDRGDWASISEEGRNYMRKLLDVANSPRKGDLEAFGGITPKAKGIPAEDAFAGIGKAGKVGTELPQSTGFKLDGVEQQEPAVPFTKDFYDKHGTTVEEYENRSTFFGIKDTTSAELHNSLLGVAFNAARFDDSFDIFKDAITPTRWNSTTWTPEQLERIRNEVKDPNYINVVTGGDPENLDDLIKLANENAAMAAKTRGAGVGAQLVGGIVGAGVDPLSYVPLVGVAGKGLKVVNKAFVVGAQSAALGAVSEAVRTSVAGGEAHYQDAILGGLMFGAGMSAIADGVSAALGRVRAGQVSNDFDRMATRLEAREAAFQAGGEDLTRMPTDGRSFDGEHAGVNYSPLETEPGAVVLPQGQILSDTNPINPQTLQEFEQVNPERAARGISLGGFAEISLKTLRSENETVRSIASDLVRSPTGMESGSNGKFGSTASDIKERLHSLNQRTYNLLYDAVRIAMKDPEFSTGGVKMSRKEVRQEIYKRAALAIERPELQSGLTKGERKVMDILKQHFDLKRELMENPAIFGNVKARSIFPGSRHNGTYVPNVYDRAIKLYMVSRYGNEGLQRAIAESWLTSYRARPEVKARVDEYLMELNGLKTVQEVTPDMVQKHAMDKAYGISHTDQFTSSSIIDENLEGLVGIENNNFLEARNMFDSDMAVTLPDGSTFSVNDLRNYDMADILPAYDRRVDGDVAIMGGSGKTTKELKDEIMALDKQSEGNGSLKGEVEALKDTVKILTGRARMNPEGAFGTILRSMTDLAFFAKNFYMPFQNFTEVAAMLSKGNTSAVLHGIPLLNDWVSRRKPMSGSEIKEIHGMVFGKELDQLMRPGREDHIRRLRESTDTNTVVANVVGTIRFGTQELSARSPWTMLLNGTSNYIIDAARQGVLGDVAGAALAGKGSKFGKANYLKSASVSPEQWKGIKQLFRDHATRDADGKFTIRDKQAFANDPRTMDLWRLADKVADETILRPHKVSSQDTKAYGAAVKMVMQFKNFVIKSLNSRFVRLFYDATKNNRAIDS | ||||||
Topological domain | 1159-1322 | Cytoplasmic | ||||
Sequence: KAYSLQEHKRKEYLKNALDPKMIAHASLSRSSHLGSPLSVYDMIGGLFGNDTYQYTRSTVLPKEKTERDRNKAWTGKEVAGNIVGAISNQVPAVGLVGNIGSTAMNASTLLSSDGKQSEVEFRTGVFNSMRELVPNDPLTQQLIMHIFEEQGIKIKDDATRPRQ |
Keywords
- Cellular component
Interaction
Subunit
Homotetramer. Interacts with gp15; after ejection the gp15-gp16 complex composed of a gp15 octamer and a gp16 tetramer probably binds both the viral DNA and the host inner membrane.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 14-128 | Transglycosylase SLT | ||||
Sequence: LFQKAADEHGVSYDLLRKLAFNESGFNPKAKSPTGPLGLMQFTKGTAAGLGLKVTGGDDDERFNPELAVDAAARHLSDLVRKYDGDELKAALAYNQGEGRNGAPQMQAYDRGDWA | ||||||
Region | 23-110 | Transglycosylase SLT-type domain | ||||
Sequence: GVSYDLLRKLAFNESGFNPKAKSPTGPLGLMQFTKGTAAGLGLKVTGGDDDERFNPELAVDAAARHLSDLVRKYDGDELKAALAYNQG |
Domain
The N-terminus contains the transglycosylase activity. The C-terminus is essential for the viral DNA translocation into the host cytoplasm.
Sequence similarities
Belongs to the transglycosylase Slt family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,322
- Mass (Da)144,382
- Last updated2022-12-14 v1
- Checksum198AD4393B29B099