A0A8X8M5D0 · A0A8X8M5D0_9CAUD

  • Protein
    Peptidoglycan transglycosylase gp16
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Component of the cylindrical core that assembles on the inner surface of the capsid during capsid formation and plays a role in viral DNA ejection into the host cell. The inner core is composed of stacked rings of gp14, gp15 and gp16 proteins. Following binding to the host cell surface, the internal core is disassembled and gp16 is ejected along with gp14 and gp15 into the infected cell. Gp16 probably inserts in the host inner membrane and remains associated with gp15. The gp15-gp16 complex binds to both the viral DNA and the host inner membrane, probably escorting the leading end of the genome through the periplasm and controlling the extent of DNA translocated into the host cell. Functions as an exolysin that catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine residues in peptidoglycans allowing the local digestion of the bacterial peptidoglycan wall.

Catalytic activity

  • Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.
    EC:4.2.2.n1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site.

113221002003004005006007008009001,0001,1001,2001,300
TypeIDPosition(s)Description
Active site36

GO annotations

AspectTerm
Cellular Componenthost cell plasma membrane
Cellular Componentmembrane
Cellular Componentvirion component
Molecular Functionhydrolase activity
Molecular Functionlytic transglycosylase activity
Biological Processdefense response to bacterium
Biological Processkilling of cells of another organism
Biological Processpeptidoglycan metabolic process
Biological Processsymbiont entry into host cell via disruption of host cell envelope
Biological Processsymbiont entry into host cell via disruption of host cell wall peptidoglycan
Biological Processsymbiont genome ejection through host cell envelope, short tail mechanism

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Peptidoglycan transglycosylase gp16
  • EC number
  • Alternative names
    • Internal core protein gp16

Organism names

  • Taxonomic identifier
  • Organism
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Autographiviridae > Studiervirinae > Teetrevirus

Accessions

  • Primary accession
    A0A8X8M5D0

Proteomes

Subcellular Location

Virion
Host cell inner membrane
; Single-pass membrane protein
Note: The gp15-gp16 complex spans the periplasm and the cytoplasmic membrane.

Features

Showing features for topological domain.

TypeIDPosition(s)Description
Topological domain1-1137Periplasmic
Topological domain1159-1322Cytoplasmic

Keywords

Interaction

Subunit

Homotetramer. Interacts with gp15; after ejection the gp15-gp16 complex composed of a gp15 octamer and a gp16 tetramer probably binds both the viral DNA and the host inner membrane.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain14-128Transglycosylase SLT
Region23-110Transglycosylase SLT-type domain

Domain

The N-terminus contains the transglycosylase activity. The C-terminus is essential for the viral DNA translocation into the host cytoplasm.

Sequence similarities

Belongs to the transglycosylase Slt family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,322
  • Mass (Da)
    144,382
  • Last updated
    2022-12-14 v1
  • Checksum
    198AD4393B29B099
MSYDKTKPSEYDGLFQKAADEHGVSYDLLRKLAFNESGFNPKAKSPTGPLGLMQFTKGTAAGLGLKVTGGDDDERFNPELAVDAAARHLSDLVRKYDGDELKAALAYNQGEGRNGAPQMQAYDRGDWASISEEGRNYMRKLLDVANSPRKGDLEAFGGITPKAKGIPAEDAFAGIGKAGKVGTELPQSTGFKLDGVEQQEPAVPFTKDFYDKHGTTVEEYENRSTFFGIKDTTSAELHNSLLGVAFNAARFDDSFDIFKDAITPTRWNSTTWTPEQLERIRNEVKDPNYINVVTGGDPENLDDLIKLANENAAMAAKTRGAGVGAQLVGGIVGAGVDPLSYVPLVGVAGKGLKVVNKAFVVGAQSAALGAVSEAVRTSVAGGEAHYQDAILGGLMFGAGMSAIADGVSAALGRVRAGQVSNDFDRMATRLEAREAAFQAGGEDLTRMPTDGRSFDGEHAGVNYSPLETEPGAVVLPQGQILSDTNPINPQTLQEFEQVNPERAARGISLGGFAEISLKTLRSENETVRSIASDLVRSPTGMESGSNGKFGSTASDIKERLHSLNQRTYNLLYDAVRIAMKDPEFSTGGVKMSRKEVRQEIYKRAALAIERPELQSGLTKGERKVMDILKQHFDLKRELMENPAIFGNVKARSIFPGSRHNGTYVPNVYDRAIKLYMVSRYGNEGLQRAIAESWLTSYRARPEVKARVDEYLMELNGLKTVQEVTPDMVQKHAMDKAYGISHTDQFTSSSIIDENLEGLVGIENNNFLEARNMFDSDMAVTLPDGSTFSVNDLRNYDMADILPAYDRRVDGDVAIMGGSGKTTKELKDEIMALDKQSEGNGSLKGEVEALKDTVKILTGRARMNPEGAFGTILRSMTDLAFFAKNFYMPFQNFTEVAAMLSKGNTSAVLHGIPLLNDWVSRRKPMSGSEIKEIHGMVFGKELDQLMRPGREDHIRRLRESTDTNTVVANVVGTIRFGTQELSARSPWTMLLNGTSNYIIDAARQGVLGDVAGAALAGKGSKFGKANYLKSASVSPEQWKGIKQLFRDHATRDADGKFTIRDKQAFANDPRTMDLWRLADKVADETILRPHKVSSQDTKAYGAAVKMVMQFKNFVIKSLNSRFVRLFYDATKNNRAIDSALTAIISMGLAGGYYVAQAHLKAYSLQEHKRKEYLKNALDPKMIAHASLSRSSHLGSPLSVYDMIGGLFGNDTYQYTRSTVLPKEKTERDRNKAWTGKEVAGNIVGAISNQVPAVGLVGNIGSTAMNASTLLSSDGKQSEVEFRTGVFNSMRELVPNDPLTQQLIMHIFEEQGIKIKDDATRPRQ

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
ON528724
EMBL· GenBank· DDBJ
URY11117.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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