A0A8X7NR02 · A0A8X7NR02_CANPA
- ProteinSulfate adenylyltransferase
- GeneMET3
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids522 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the first intracellular reaction of sulfate assimilation, forming adenosine-5'-phosphosulfate (APS) from inorganic sulfate and ATP. Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids.
Catalytic activity
- sulfate + ATP + H+ = adenosine 5'-phosphosulfate + diphosphate
Pathway
Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 205 | sulfate (UniProtKB | ChEBI) | |||
Binding site | 205-208 | ATP (UniProtKB | ChEBI) | |||
Active site | 206 | ||||
Active site | 207 | ||||
Binding site | 207 | sulfate (UniProtKB | ChEBI) | |||
Active site | 208 | ||||
Site | 211 | Transition state stabilizer | |||
Site | 214 | Transition state stabilizer | |||
Binding site | 301-304 | ATP (UniProtKB | ChEBI) | |||
Binding site | 305 | sulfate (UniProtKB | ChEBI) | |||
Site | 340 | Induces change in substrate recognition on ATP binding | |||
Binding site | 343 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | sulfate adenylyltransferase (ATP) activity | |
Biological Process | cysteine biosynthetic process | |
Biological Process | methionine biosynthetic process | |
Biological Process | sulfate assimilation via adenylyl sulfate reduction | |
Biological Process | sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSulfate adenylyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Debaryomycetaceae > Candida/Lodderomyces clade > Candida
Accessions
- Primary accessionA0A8X7NR02
Proteomes
Subcellular Location
Interaction
Subunit
Homohexamer. Dimer of trimers.
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-175 | N-terminal | |||
Domain | 5-170 | ATP-sulfurylase PUA-like | |||
Domain | 180-398 | Sulphate adenylyltransferase catalytic | |||
Region | 406-522 | Required for oligomerization; adenylyl-sulfate kinase-like | |||
Domain
The oligomerization domain is distantly related to APS kinases, but it is not functional and does not bind APS. It is required for oligomerization of the enzyme, although the oligomerization state has no effect on the catalytic activity of the enzyme.
Sequence similarities
Belongs to the sulfate adenylyltransferase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length522
- Mass (Da)58,883
- Last updated2022-12-14 v1
- Checksum055E0D0FF0AA6471
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JABWAB010000001 EMBL· GenBank· DDBJ | KAF6058767.1 EMBL· GenBank· DDBJ | Genomic DNA |