A0A8V0ZL83 · A0A8V0ZL83_CHICK

  • Protein
    Phosphatidylserine decarboxylase proenzyme, mitochondrial
  • Gene
    PISD
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine. May be involved in lipid droplet biogenesis at the endoplasmic reticulum membrane.

Catalytic activity

Cofactor

pyruvate (UniProtKB | Rhea| CHEBI:15361 )

Note: Binds 1 pyruvoyl group covalently per subunit.

Pathway

Lipid metabolism.
Phospholipid metabolism; phosphatidylethanolamine biosynthesis.

Features

Showing features for active site, site.

Type
IDPosition(s)Description
Active site195Charge relay system; for autoendoproteolytic cleavage activity
Active site271Charge relay system; for autoendoproteolytic cleavage activity
Site381-382Cleavage (non-hydrolytic); by autocatalysis
Active site382Charge relay system; for autoendoproteolytic cleavage activity
Active site382Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity

GO annotations

AspectTerm
Cellular Componentlipid droplet
Cellular Componentmitochondrial inner membrane
Molecular Functionphosphatidylserine decarboxylase activity
Biological Processphosphatidylethanolamine biosynthetic process
Biological Processprotein autoprocessing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

Gene names

    • Name
      PISD

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Broiler
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Galloanserae > Galliformes > Phasianidae > Phasianinae > Gallus

Accessions

  • Primary accession
    A0A8V0ZL83

Proteomes

Subcellular Location

Phosphatidylserine decarboxylase alpha chain

Mitochondrion inner membrane
; Peripheral membrane protein
Note: Anchored to the mitochondrial inner membrane through its interaction with the integral membrane beta chain.

Phosphatidylserine decarboxylase beta chain

Mitochondrion inner membrane
; Single-pass membrane protein

Features

Showing features for topological domain.

TypeIDPosition(s)Description
Topological domain1-80Mitochondrial matrix
Topological domain100-413Mitochondrial intermembrane

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

Type
IDPosition(s)Description
ChainPRO_50365213571-381Phosphatidylserine decarboxylase beta chain
Modified residue382Pyruvic acid (Ser); by autocatalysis
ChainPRO_5036521356382-413Phosphatidylserine decarboxylase alpha chain

Post-translational modification

Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase.

Keywords

Interaction

Subunit

Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.

Family & Domains

Features

Showing features for compositional bias, region.

Type
IDPosition(s)Description
Compositional bias21-38Polar residues
Region21-47Disordered

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    413
  • Mass (Da)
    46,681
  • Last updated
    2022-12-14 v1
  • Checksum
    45EE1756599BD400
MCQSNALQGPDLHTGKWLRSGNGGSSSCAGNPQPQLESPGPAGSAGGTPNRRARFRLQFPQLALRRRLGQLSCMSRPALKLRSWPLTILYYLLPFGALKPLTRVGWRPVSRVALYKSVPTRLLSRAWGRLNQVELPTWLRKPVYSLYIWTFGVNMKEAAVEDLHHYRNLSEFFRRKLKPQARPVCCVHSVISPSDGKILNFGQVKNCEVEQVKGVTYSLESFLGPRISTEDFRFSEVPPGNSFQQQLVTKEGNELYHCVIYLAPGDYHCFHSPTDWRVSHRRHFPGSLMSVNPGVARWIKELFCHNERVVLTGDWKHGFFSLTAVGATNVGSIRIYFDQDLHTNSPRYSKGSYNDFSFISNNNKEGIPMRKGEHLGEFNLGSTIVLIFEAPKDFKFHLKAGQKIRFGEALGSL

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8V0ZTP0A0A8V0ZTP0_CHICKPISD423

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias21-38Polar residues

Keywords

Genome annotation databases

Similar Proteins

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