A0A8T3X464 · A0A8T3X464_9ARCH
- ProteintRNA-splicing ligase RtcB
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids155 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Essential for tRNA splicing and maturation. Acts by directly joining spliced tRNA halves to mature-sized tRNAs. Joins RNA with 2',3'-cyclic-phosphate or 3'-phosphate ends to RNA with 5'-hydroxy ends.
Catalytic activity
- a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate + H+
Cofactor
Note: Binds 2 manganese ions per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 3 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 3-4 | GMP (UniProtKB | ChEBI) | ||||
Sequence: HN | ||||||
Binding site | 52-55 | GMP (UniProtKB | ChEBI) | ||||
Sequence: GGSM | ||||||
Binding site | 59 | GMP (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Active site | 78 | GMP-histidine intermediate | ||||
Sequence: H | ||||||
Binding site | 78-81 | GMP (UniProtKB | ChEBI) | ||||
Sequence: HGSG | ||||||
Binding site | 154 | GMP (UniProtKB | ChEBI) | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | GTP binding | |
Molecular Function | metal ion binding | |
Molecular Function | RNA ligase (ATP) activity | |
Biological Process | RNA processing |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-splicing ligase RtcB
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Candidatus Woesearchaeota
Accessions
- Primary accessionA0A8T3X464
Proteomes
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 71-98 | Disordered | ||||
Sequence: ETFGSTAHGSGRTMSRNQAKHQWRGEQL | ||||||
Compositional bias | 76-92 | Polar residues | ||||
Sequence: TAHGSGRTMSRNQAKHQ |
Sequence similarities
Belongs to the RtcB family.
Family and domain databases
Sequence
- Sequence statusFragment
- Length155
- Mass (Da)16,867
- Last updated2022-10-12 v1
- Checksum84134660295E1C29
Features
Showing features for non-terminal residue, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: V | ||||||
Compositional bias | 76-92 | Polar residues | ||||
Sequence: TAHGSGRTMSRNQAKHQ |