A0A8S9ZEE4 · A0A8S9ZEE4_9BILA
- ProteinAnamorsin homolog
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids758 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis, facilitating the de novo assembly of a [4Fe-4S] cluster on the cytosolic Fe-S scaffold complex. Electrons are transferred from NADPH via a FAD- and FMN-containing diflavin oxidoreductase. Together with the diflavin oxidoreductase, also required for the assembly of the diferric tyrosyl radical cofactor of ribonucleotide reductase (RNR), probably by providing electrons for reduction during radical cofactor maturation in the catalytic small subunit.
Cofactor
Protein has several cofactor binding sites:
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 383 | [2Fe-2S] cluster (UniProtKB | ChEBI) | |||
Binding site | 395 | [2Fe-2S] cluster (UniProtKB | ChEBI) | |||
Binding site | 398 | [2Fe-2S] cluster (UniProtKB | ChEBI) | |||
Binding site | 400 | [2Fe-2S] cluster (UniProtKB | ChEBI) | |||
Binding site | 422 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 425 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 433 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 436 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
GO annotations
Keywords
- Ligand
Names & Taxonomy
Protein names
- Recommended nameAnamorsin homolog
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Tylenchina > Tylenchomorpha > Tylenchoidea > Meloidogynidae > Meloidogyninae > Meloidogyne
Accessions
- Primary accessionA0A8S9ZEE4
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 27-45 | Helical | |||
Keywords
- Cellular component
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for coiled coil, region, motif, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Coiled coil | 51-169 | ||||
Region | 208-227 | Disordered | |||
Motif | 422-425 | Cx2C motif 1 | |||
Region | 422-436 | Fe-S binding site B | |||
Motif | 433-436 | Cx2C motif 2 | |||
Domain | 589-751 | 3'-5' exonuclease | |||
Domain
The C-terminal domain binds 2 Fe-S clusters but is otherwise mostly in an intrinsically disordered conformation.
The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine. It is required for correct assembly of the 2 Fe-S clusters.
The twin Cx2C motifs are involved in the recognition by the mitochondrial MIA40-ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space.
Sequence similarities
Belongs to the anamorsin family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length758
- Mass (Da)85,621
- Last updated2022-10-12 v1
- ChecksumA3A261A1B90E0822
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JABEBT010000130 EMBL· GenBank· DDBJ | KAF7630784.1 EMBL· GenBank· DDBJ | Genomic DNA |