A0A8L2Q612 · A0A8L2Q612_RAT
- ProteinDNA repair nuclease/redox regulator APEX1
- GeneApex1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids308 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.
Catalytic activity
Cofactor
Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Probably binds two magnesium or manganese ions per subunit.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 58 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 86 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 161 | |||||
Sequence: Y | ||||||
Active site | 200 | Proton donor/acceptor | ||||
Sequence: D | ||||||
Binding site | 200 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 202 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Site | 202 | Transition state stabilizer | ||||
Sequence: N | ||||||
Site | 273 | Important for catalytic activity | ||||
Sequence: D | ||||||
Binding site | 298 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 299 | Proton acceptor | ||||
Sequence: H | ||||||
Binding site | 299 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Site | 299 | Interaction with DNA substrate | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Cellular Component | nucleus | |
Molecular Function | DNA binding | |
Molecular Function | endonuclease activity | |
Molecular Function | exonuclease activity | |
Molecular Function | metal ion binding | |
Biological Process | DNA recombination | |
Biological Process | DNA repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA repair nuclease/redox regulator APEX1
- EC number
- Short namesAPEN ; REF-1
- Alternative names
- Cleaved into 1 chains
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionA0A8L2Q612
Proteomes
Organism-specific databases
Subcellular Location
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-47 | Disordered | ||||
Sequence: MNRIAPLPPEPETKKSKGAAKKTEKEAAGEGPVLYEDPPDQKTSASG | ||||||
Compositional bias | 14-31 | Basic and acidic residues | ||||
Sequence: KKSKGAAKKTEKEAAGEG | ||||||
Domain | 55-299 | Endonuclease/exonuclease/phosphatase | ||||
Sequence: CSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPAELQELPGLTHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGEEEHDQEGRVIVAEFESFILVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKDLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGEMLQAVPLADSFRHLYPNTAYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDH |
Sequence similarities
Belongs to the DNA repair enzymes AP/ExoA family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length308
- Mass (Da)34,590
- Last updated2022-08-03 v1
- ChecksumAA051A975629586F
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
P43138 | APEX1_RAT | Apex1 | 317 | ||
A0A8L2QZQ5 | A0A8L2QZQ5_RAT | Apex1 | 316 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 14-31 | Basic and acidic residues | ||||
Sequence: KKSKGAAKKTEKEAAGEG |
Keywords
- Technical term