A0A8K0P4Y3 · A0A8K0P4Y3_LADFU
- ProteinProtein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids572 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Participates in O-mannosyl glycosylation by catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties. Is specific for alpha linked terminal mannose.
Catalytic activity
- 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl)-L-threonyl-[protein] + UDP + H+
Cofactor
Note: The manganese ion interacts primarily with the substrate UDP-N-acetylglucosamine.
Pathway
Protein modification; protein glycosylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Golgi membrane | |
Molecular Function | beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity | |
Molecular Function | carbohydrate binding | |
Molecular Function | manganese ion binding | |
Biological Process | O-glycan processing |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase
- EC number
- Short namesPOMGnT1
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Palaeoptera > Odonata > Epiprocta > Anisoptera > Libellulidae > Ladona
Accessions
- Primary accessionA0A8K0P4Y3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Single-pass type II membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 44-65 | Helical | |||
Keywords
- Cellular component
PTM/Processing
Keywords
- PTM
Interaction
Subunit
Interacts with DAG1 (via O-linked mannose moiety). Interacts (via transmembrane domain) with FKTN; the interaction is direct and is required for normal location in Golgi membranes.
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-22 | Disordered | |||
Domain | 136-225 | ILEI/PANDER | |||
Domain
The stem domain mediates specific interaction with beta-linked N-acetylglucosamine moieties of O-glycosylated proteins. It also interacts with its product, N-acetyl-beta-D-glucosaminyl-(1->2)-O-alpha-D-mannosylprotein.
Sequence similarities
Belongs to the glycosyltransferase 13 family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusFragment
- Length572
- Mass (Da)65,830
- Last updated2022-08-03 v1
- Checksum5B6B0537D12E2CF3
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Non-terminal residue | 1 | ||||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KZ308989 EMBL· GenBank· DDBJ | KAG8236090.1 EMBL· GenBank· DDBJ | Genomic DNA |