A0A8J8W2U8 · A0A8J8W2U8_9EURO
- ProteinNADPH-dependent diflavin oxidoreductase 1
- GeneTAH18
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids635 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of DRE2, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. Positively controls H2O2-induced cell death.
Catalytic activity
- 2 oxidized [2Fe-2S]-[protein] + NADPH = 2 reduced [2Fe-2S]-[protein] + NADP+ + H+
Cofactor
Protein has several cofactor binding sites:
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 18-23 | FMN (UniProtKB | ChEBI) | |||
Binding site | 65-68 | FMN (UniProtKB | ChEBI) | |||
Binding site | 103-112 | FMN (UniProtKB | ChEBI) | |||
Binding site | 369 | FAD (UniProtKB | ChEBI) | |||
Binding site | 399-402 | FAD (UniProtKB | ChEBI) | |||
Binding site | 438-441 | FAD (UniProtKB | ChEBI) | |||
Binding site | 480 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 548-549 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 554-558 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 635 | FAD (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | mitochondrion | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | NADP binding | |
Molecular Function | NADPH-hemoprotein reductase activity | |
Biological Process | iron-sulfur cluster assembly |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADPH-dependent diflavin oxidoreductase 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Penicillium
Accessions
- Primary accessionA0A8J8W2U8
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Relocalizes to mitochondria after H2O2 exposure.
Keywords
- Cellular component
Interaction
Subunit
Interacts with DRE2; as part of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery.
Structure
Family & Domains
Features
Showing features for domain, compositional bias, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 12-156 | Flavodoxin-like | |||
Compositional bias | 196-213 | Polar residues | |||
Region | 196-216 | Disordered | |||
Domain | 220-466 | FAD-binding FR-type | |||
Sequence similarities
Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
In the N-terminal section; belongs to the flavodoxin family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length635
- Mass (Da)71,436
- Last updated2022-05-25 v1
- ChecksumAEEB00059D9407FA
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 196-213 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
WIWV01000040 EMBL· GenBank· DDBJ | KAF7716494.1 EMBL· GenBank· DDBJ | Genomic DNA |