A0A8J7FAS6 · A0A8J7FAS6_9GAMM

  • Protein
    tRNA-dihydrouridine(20/20a) synthase
  • Gene
    dusA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

FMN (UniProtKB | Rhea| CHEBI:58210 )

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site53FMN (UniProtKB | ChEBI)
Site80Interacts with tRNA
Active site83Proton donor
Binding site122FMN (UniProtKB | ChEBI)
Binding site154FMN (UniProtKB | ChEBI)
Site166Interacts with tRNA; defines subfamily-specific binding signature
Site169Interacts with tRNA
Binding site194-196FMN (UniProtKB | ChEBI)
Binding site216-217FMN (UniProtKB | ChEBI)
Site282Interacts with tRNA; defines subfamily-specific binding signature
Site285Interacts with tRNA; defines subfamily-specific binding signature

GO annotations

AspectTerm
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular FunctiontRNA binding
Molecular FunctiontRNA dihydrouridine synthase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-dihydrouridine(20/20a) synthase
  • EC number
  • Alternative names
    • U20-specific dihydrouridine synthase
      (U20-specific Dus
      )
    • tRNA-dihydrouridine synthase A

Gene names

    • Name
      dusA
    • ORF names
      IOQ59_09620

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • N1Y112
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Oceanospirillales > Oceanospirillaceae > Pontibacterium

Accessions

  • Primary accession
    A0A8J7FAS6

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-305DUS-like FMN-binding

Sequence similarities

Belongs to the Dus family. DusA subfamily.
Belongs to the dus family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    311
  • Mass (Da)
    35,675
  • Last updated
    2022-05-25 v1
  • Checksum
    BBE4403587129C95
MMDWTDRHCRYFHRLISHHAVLYTEMVTTGALIHADEARFLQFDPKEQPVALQLGGSNPEDLTQCTKMAEDWGYNEVNLNVGCPSDRVQNNMIGACLMAHPQLVAECLHKMQSVVDIPVTVKHRLGIDDMDSYEELHNFVDIVKQSGCRTFIVHARKAILQGLSPKENRDIPPLKYEWVYQLKREFPELEIIINGGIKTLEECEQHLQQVDGVMVGREAYQNPYMMAEVDNRLYDADSTPQQRKAVIEAMLPYIDAQLNDGRYLNHVVRHLLGLFHAQRGGRQFRRYLSENAHKPGASINTLKEALDLIPD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JADEYS010000008
EMBL· GenBank· DDBJ
MBE9397517.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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