A0A8J6GMV1 · A0A8J6GMV1_MICOH

Function

function

NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Modulates ketogenesis through the desuccinylation and activation of HMGCS2. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as Uox.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site96-115NAD+ (UniProtKB | ChEBI)
Binding site140substrate
Binding site143substrate
Binding site178-181NAD+ (UniProtKB | ChEBI)
Active site196Proton acceptor
Binding site204Zn2+ (UniProtKB | ChEBI)
Binding site207Zn2+ (UniProtKB | ChEBI)
Binding site245Zn2+ (UniProtKB | ChEBI)
Binding site250Zn2+ (UniProtKB | ChEBI)
Binding site287-289NAD+ (UniProtKB | ChEBI)
Binding site313-315NAD+ (UniProtKB | ChEBI)
Binding site351NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentmitochondrion
Cellular Componentnucleolus
Molecular FunctionNAD+ binding
Molecular FunctionNAD-dependent protein lysine deacetylase activity
Molecular Functionprotein-malonyllysine demalonylase activity
Molecular Functionprotein-succinyllysine desuccinylase activity
Molecular FunctionRNA binding
Molecular Functiontransferase activity
Molecular Functionzinc ion binding
Biological Processprotein deacetylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NAD-dependent protein deacylase sirtuin-5, mitochondrial
  • EC number
  • Alternative names
    • Regulatory protein SIR2 homolog 5
    • SIR2-like protein 5

Gene names

    • Name
      SIRT5
    • ORF names
      LTLLF_137300

Organism names

  • Taxonomic identifier
  • Strain
    • LTLLF
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Cricetidae > Arvicolinae > Microtus

Accessions

  • Primary accession
    A0A8J6GMV1

Proteomes

Subcellular Location

Mitochondrion
Cytoplasm, cytosol
Nucleus
Note: Mainly mitochondrial. Also present extramitochondrially, with a fraction present in the cytosol and very small amounts also detected in the nucleus.

Keywords

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-17
ChainPRO_503520775818-551NAD-dependent protein deacylase sirtuin-5, mitochondrial

Interaction

Subunit

Monomer. Homodimer. Interacts with CPS1.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain71-335Deacetylase sirtuin-type
Region416-465Disordered
Region531-551Disordered

Domain

In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-140 and Arg-143) that bind to malonylated and succinylated substrates and define the specificity.

Sequence similarities

Belongs to the sirtuin family. Class III subfamily.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    551
  • Mass (Da)
    60,872
  • Last updated
    2022-05-25 v1
  • Checksum
    A0656F7515445BB6
MAAATPLTLCPVLLGWAVSILTTKRSSLLEKQYFKETLMRLLQILPGRFVSQLCCGLKPPASPRSKICLIMARPSSNMADFRKCFANAKHIAIISGAGVSAESGVPTFRGAGGYWRKWQAQDLATPQAFARNPSQVWEFYHYRREVMKSKEPNPGHLAIAQCEARLRDQGRRVVVITQNIDELHRKAGTKNLLEIHGTLFKTRCTSCGNVAENYKSPICPALAGKGAPEPETQDARIPVDKLPRCEEAGCGGLLRPHVVWFGENLDPAVLEEVDRELALCDLCLVVGTSSVVYPAAMFAPQVASRGVPVAEFNTETTPATNIFSCYGREEYHDLQAGSLFHYMGVSIELTIFRVATPTGSPALLPRTRFSASRGLRTNRGRGEREARTASGCFRVKGQTAFFPLAMVQLRPRPSRVPAPAEAMVDEDQPASEEEEAEHGLLLGQPRSGAAAEPLDEDEDGDDEAPEELTFAHAQAEAREEELRVRESARSLKKSPGNVKVNKFLSLNNKRLPVKRAAAQFLNGTWGAKKQQNAKRFKKRWMAKKMKKKSYK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAATJU010021400
EMBL· GenBank· DDBJ
KAH0513762.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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