A0A8J6BQ15 · A0A8J6BQ15_ZIZPA
- ProteinDNA helicase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1550 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Can play two different roles depending on the source of dihydrofolate: de novo synthesis of tetrahydrofolate or recycling of the dihydrofolate released as one of the end products of the TS catalyzed reaction. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Catalytic activity
- (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-dihydrofolate + dTMP
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 1467 | |||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | MCM complex | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | dihydrofolate reductase activity | |
Molecular Function | DNA binding | |
Molecular Function | helicase activity | |
Molecular Function | hydrolase activity | |
Molecular Function | metal ion binding | |
Molecular Function | thymidylate synthase activity | |
Biological Process | DNA replication initiation | |
Biological Process | DNA unwinding involved in DNA replication | |
Biological Process | dTMP biosynthetic process | |
Biological Process | tetrahydrofolate biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA helicase
- EC number
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Zizaniinae > Zizania
Accessions
- Primary accessionA0A8J6BQ15
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-40 | Polar residues | ||||
Sequence: MDDSENNAPSTPGSPGFSTDRLPPNTTTSRGATDPSSYSD | ||||||
Region | 1-61 | Disordered | ||||
Sequence: MDDSENNAPSTPGSPGFSTDRLPPNTTTSRGATDPSSYSDDDNNSAEEAEVDPNVLPEDDG | ||||||
Compositional bias | 41-61 | Acidic residues | ||||
Sequence: DDNNSAEEAEVDPNVLPEDDG | ||||||
Region | 153-220 | Disordered | ||||
Sequence: NFRRPKRHRANFRPPREPRTPRSDDDGDGATPSSPGRSQRGMYSGGDVPMTDQTDDDPYEDEFDEEDE | ||||||
Compositional bias | 205-220 | Acidic residues | ||||
Sequence: QTDDDPYEDEFDEEDE | ||||||
Domain | 524-730 | MCM | ||||
Sequence: ISERIVKSIAPSIYGHEDIKTAIALAMFGGQEKNVRGKHRLRGDINVLLLGDPGTAKSQFLKYVEKTGHRAVYTTGKGASAVGLTAAVHKDPVTREWTLEGGALVLADRGICLIDEFDKMNDQDRVSIHEAMEQQSISISKAGIVTSLQARCSVIAAANPLGGRYDSSKTFTQNVELTDPIISRFDVLCVVKDIVDPFTDEMLARFV | ||||||
Domain | 1081-1258 | DHFR | ||||
Sequence: NYQVVVAATCDGGIGKDGDLPWKLPGDLKFFKDITRTTVDPTKKNAVIMGRKSWESLPLQARPLSGRLNIILTRSGSFDFATVENVVICGSMNSALELLASTPYCLSIEKVFVIGGGQILREYLNGPSCEAIHITDIQSSMECDTFIPPIDLSVFQPWYSSLPVVENNIRHSFVTFVR |
Sequence similarities
In the C-terminal section; belongs to the thymidylate synthase family.
In the N-terminal section; belongs to the dihydrofolate reductase family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,550
- Mass (Da)174,157
- Last updated2022-05-25 v1
- Checksum78813C325E97DE59
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8J6BKL4 | A0A8J6BKL4_ZIZPA | GUJ93_ZPchr0011g27389 | 1396 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-40 | Polar residues | ||||
Sequence: MDDSENNAPSTPGSPGFSTDRLPPNTTTSRGATDPSSYSD | ||||||
Compositional bias | 41-61 | Acidic residues | ||||
Sequence: DDNNSAEEAEVDPNVLPEDDG | ||||||
Compositional bias | 205-220 | Acidic residues | ||||
Sequence: QTDDDPYEDEFDEEDE |
Keywords
- Technical term