A0A8J4WN35 · A0A8J4WN35_9BACT
- ProteinAdenylosuccinate synthetase
- GenepurA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids422 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.
Catalytic activity
- IMP + L-aspartate + GTP = N6-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H+
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 12-18 | GTP (UniProtKB | ChEBI) | |||
Active site | 13 | Proton acceptor | |||
Binding site | 13 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 13-16 | IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 38-41 | IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 40 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 40-42 | GTP (UniProtKB | ChEBI) | |||
Active site | 41 | Proton donor | |||
Binding site | 129 | IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Active site | 140 | ||||
Binding site | 143 | IMP (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
Binding site | 223 | IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 238 | IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 297-303 | substrate | |||
Binding site | 301 | IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 303 | GTP (UniProtKB | ChEBI) | |||
Binding site | 329-331 | GTP (UniProtKB | ChEBI) | |||
Binding site | 411-413 | GTP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | adenylosuccinate synthase activity | |
Molecular Function | GTP binding | |
Molecular Function | magnesium ion binding | |
Biological Process | 'de novo' AMP biosynthetic process | |
Biological Process | IMP metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAdenylosuccinate synthetase
- EC number
- Short namesAMPSase ; AdSS
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Verrucomicrobiota > Spartobacteria > Chthoniobacterales > Chthoniobacteraceae > Candidatus Udaeobacter
Accessions
- Primary accessionA0A8J4WN35
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length422
- Mass (Da)46,040
- Last updated2022-05-25 v1
- ChecksumF497BB16BBB44558
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JAALOD010000022 EMBL· GenBank· DDBJ | KAF5408591.1 EMBL· GenBank· DDBJ | Genomic DNA |