A0A8J4WMH2 · A0A8J4WMH2_9BACT

Function

function

Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Features

Showing features for site, binding site.

159050100150200250300350400450500550
Type
IDPosition(s)Description
Site31Important for tRNA non-discrimination
Site83Important for tRNA non-discrimination
Binding site174L-aspartate (UniProtKB | ChEBI)
Binding site220L-aspartate (UniProtKB | ChEBI)
Binding site220-222ATP (UniProtKB | ChEBI)
Binding site229ATP (UniProtKB | ChEBI)
Binding site448L-aspartate (UniProtKB | ChEBI)
Binding site483ATP (UniProtKB | ChEBI)
Binding site490L-aspartate (UniProtKB | ChEBI)
Binding site535-538ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionaspartate-tRNA ligase activity
Molecular FunctionATP binding
Molecular Functionnucleic acid binding
Biological Processaspartyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Aspartate--tRNA(Asp/Asn) ligase
  • EC number
  • Alternative names
    • Aspartyl-tRNA synthetase
      (AspRS
      )
    • Non-discriminating aspartyl-tRNA synthetase
      (ND-AspRS
      )

Gene names

    • Name
      aspS
    • ORF names
      Udaeo2_20430

Organism names

  • Taxonomic identifier
  • Strain
    • AEW3
  • Taxonomic lineage
    Bacteria > Verrucomicrobiota > Spartobacteria > Chthoniobacterales > Chthoniobacteraceae > Candidatus Udaeobacter

Accessions

  • Primary accession
    A0A8J4WMH2

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain149-556Aminoacyl-transfer RNA synthetases class-II family profile
Region198-201Aspartate

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    590
  • Mass (Da)
    66,955
  • Last updated
    2022-05-25 v1
  • Checksum
    4394BEEA47918F8C
MYRTHHCNSLRKTDVGRQVALAGWINVTRDHGGVIFIDLRDREGLTQIVFRPEENAELAKQAHTLRSEDVIQVSGRVAARLPGTENPNLATGDIEIIPSEMKILNRSDDLPFPIDTEPHNEDLRMTHRYYDLRRPRLTRNLRLRHIAAKATRDYLDSQGYVEVETPVLSKSTPEGARDFLVPSRITPGKFYALPQAPQQYKQLLMVAGVEKYFQIAKCFRDEDLRADRQPEFTQIDIEASFVTPEDIFAVTEGMLAAIFRAARGIEIKIPFDRLTYREAVDRYGSDKPNRRFGMELVGLEETFRQSSFKVFRNALDAGGVVKAINAKGFATITIGQADELTEIAKLYGAKGLAFIKIENGEWKSPIVKFFSESEKAALRSKLNIEEGDCVFFGADKWEIACEVLGRLRLRIAEMQKLTPVADVWDFLWVTDFPLFEWSAEENKWNAMHHPFTRPKAEDMSLFDEKRFPDVRAEAYDVVLNGVEIGGGSIRIYEPELQDKMFEALGISPENRQAQFGHLLRALHLGAPPHGGIAFGFDRLVMLIAGEQGIRDVIAFPKNNRGMDMMSNSPAEVDPRQLRDLGIKLTKEKKL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAALOD010000051
EMBL· GenBank· DDBJ
KAF5407826.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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