A0A8J4T9C9 · A0A8J4T9C9_9BACT
- ProteintRNA(Ile)-lysidine synthase
- GenetilS
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids355 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.
Catalytic activity
- ATP + cytidine34 in tRNA(Ile2) + L-lysine = AMP + diphosphate + H+ + lysidine34 in tRNA(Ile2)
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | ligase activity, forming carbon-nitrogen bonds | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA(Ile)-lysidine synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Verrucomicrobiota > Spartobacteria > Chthoniobacterales > Chthoniobacteraceae > Candidatus Udaeobacter
Accessions
- Primary accessionA0A8J4T9C9
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 31-87 | tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal | ||||
Sequence: YLIGVSGGRDSVVLLHWLISVGYKNLMVCHLNHLLRGRSSDADARFVQKRVEHYDHE | ||||||
Domain | 114-244 | tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal | ||||
Sequence: LHFELGTLDVRAFATKQKMSIEAAARDSRYSFFAKVAKRRRCHTIFLAHHADDLVETFLINLFRGAGSAGLAAMREVSTRCIDDVGLTIVRPLLGVWRKEIDNYVRKHRLTFREDASNKNLNPLRNRIRHR | ||||||
Domain | 288-352 | tRNA(Ile)-lysidine synthase substrate-binding | ||||
Sequence: SVATLRELPVALQRRAILKWLRAQKVSEVGFDVIERVRLLIDPTGQVAKVNLAHNRHARRRSGKI |
Domain
The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.
Sequence similarities
Belongs to the tRNA(Ile)-lysidine synthase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length355
- Mass (Da)40,445
- Last updated2022-05-25 v1
- Checksum10AF0E0B8A9385FA
Keywords
- Technical term