A0A8J4T8F3 · A0A8J4T8F3_9BACT
- Protein6,7-dimethyl-8-ribityllumazine synthase
- GeneribH
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids166 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.
Catalytic activity
- (2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O + H+
Pathway
Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 34 | 5-amino-6-(D-ribitylamino)uracil (UniProtKB | ChEBI) | |||
Binding site | 67-69 | 5-amino-6-(D-ribitylamino)uracil (UniProtKB | ChEBI) | |||
Binding site | 91-93 | 5-amino-6-(D-ribitylamino)uracil (UniProtKB | ChEBI) | |||
Binding site | 96-97 | (2S)-2-hydroxy-3-oxobutyl phosphate (UniProtKB | ChEBI) | |||
Active site | 99 | Proton donor | |||
Binding site | 124 | 5-amino-6-(D-ribitylamino)uracil (UniProtKB | ChEBI) | |||
Binding site | 138 | (2S)-2-hydroxy-3-oxobutyl phosphate (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | riboflavin synthase complex | |
Molecular Function | 6,7-dimethyl-8-ribityllumazine synthase activity | |
Biological Process | riboflavin biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name6,7-dimethyl-8-ribityllumazine synthase
- EC number
- Short namesDMRL synthase ; LS ; Lumazine synthase
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Verrucomicrobiota > Spartobacteria > Chthoniobacterales > Chthoniobacteraceae > Candidatus Udaeobacter
Accessions
- Primary accessionA0A8J4T8F3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Sequence
- Sequence statusComplete
- Length166
- Mass (Da)18,267
- Last updated2022-05-25 v1
- ChecksumFDAA4CE12EEF9562
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JAALOD010000079 EMBL· GenBank· DDBJ | KAF5407285.1 EMBL· GenBank· DDBJ | Genomic DNA |