A0A8J3HUX2 · A0A8J3HUX2_9RICK

  • Protein
    2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
  • Gene
    ispF
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

a divalent metal cation (UniProtKB | Rhea| CHEBI:60240 )

Note: Binds 1 divalent metal cation per subunit.

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site12a divalent metal cation (UniProtKB | ChEBI)
Binding site12-144-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site14a divalent metal cation (UniProtKB | ChEBI)
Site44Transition state stabilizer
Binding site44-454-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site52a divalent metal cation (UniProtKB | ChEBI)
Binding site66-684-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site142-1454-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Site143Transition state stabilizer
Binding site1524-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity
Molecular Functionmetal ion binding
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
  • EC number
  • Short names
    MECDP-synthase
    ; MECPP-synthase
    ; MECPS

Gene names

    • Name
      ispF
    • ORF names
      sL5_07150

Organism names

  • Taxonomic identifier
  • Strain
    • L5
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Rickettsiales > Anaplasmataceae > Candidatus Mesenet

Accessions

  • Primary accession
    A0A8J3HUX2

Proteomes

Interaction

Subunit

Homotrimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain5-1642-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Sequence similarities

Belongs to the IspF family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    183
  • Mass (Da)
    20,818
  • Last updated
    2022-05-25 v1
  • Checksum
    C636670AFFA26CA9
MMPKYRVGIGYDVHRFIALEDGMGSCEIKICGVPVKHDMEIEAHSDGDVGIHAVVDAMLGAMALGDIGQHFPPTDLKWKNKDSSHFLEFAVKQVRDKGYSVSNADIIIVCEKPKISQYRDNMKKRMSELLQINTNLVSIKATTTEKLDDIGRRKGIAAYAIVQVQRINWFYFFFNCCFQRLVR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BNGU01000029
EMBL· GenBank· DDBJ
GHM59722.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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