A0A8J2Z7S3 · A0A8J2Z7S3_9PROT

  • Protein
    Carbamoyl phosphate synthase small chain
  • Gene
    carA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site122L-glutamine (UniProtKB | ChEBI)
Binding site315L-glutamine (UniProtKB | ChEBI)
Binding site317L-glutamine (UniProtKB | ChEBI)
Active site344Nucleophile
Binding site345L-glutamine (UniProtKB | ChEBI)
Binding site348L-glutamine (UniProtKB | ChEBI)
Binding site386L-glutamine (UniProtKB | ChEBI)
Binding site388L-glutamine (UniProtKB | ChEBI)
Binding site389L-glutamine (UniProtKB | ChEBI)
Active site428
Active site430

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular FunctionDNA binding
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • ORF names
      GCM10010964_01320

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • CGMCC 1.16330
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Rhodospirillales > Acetobacteraceae > Caldovatus

Accessions

  • Primary accession
    A0A8J2Z7S3

Proteomes

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-258CPSase
Region55-77Disordered
Domain78-208Carbamoyl-phosphate synthase small subunit N-terminal

Sequence similarities

Belongs to the CarA family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    459
  • Mass (Da)
    48,731
  • Last updated
    2022-05-25 v1
  • Checksum
    8440987495897840
MRSVEDILALMGGPEAAAARCGVGTEAIRKWRQARAIPARHWPAVLAATGLSLADLPGAPEPESHAMPEPDSAPPAGATAALVLGDGTVLWGRGFGAPGTRVGEVCFNTGMTGYQETLTDPSYAGQIITFTFPHIGNVGANPEDIEATTPAARGLVVKQDLTEPSNWRATRHLDAWLKSHGVPGIAGVDTRALTARIRDGGAPNGVLCFPADGRFDLAALRREARAWPGLVGMDLAREVSCRQSYRWDETEWVWPRGYGRQTAPRHRVVAVDYGAKRNILRCLASAGCEVIVVPATATAEEILGHAPDGVFLSNGPGDPAATAEYAVPAIRGVLERGVPLFGICLGHQLLALALGARTYKMERGHRGANQPVKDLATGKVEITSQNHGFAVDEKTLPPSAKVTHVSLFDGSNEGIACTDRPAFSVQYHPEASPGPSDSHYLFHRFVALIERHKGGSARA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BMKS01000001
EMBL· GenBank· DDBJ
GGG16748.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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