A0A8J2HQJ7 · A0A8J2HQJ7_COTCN

Function

function

ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the microtubule organizing center following nucleation.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers.
    EC:5.6.1.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for binding site.

1722100200300400500600700
TypeIDPosition(s)Description
Binding site488-495ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcentrosome
Cellular Componentcytoplasm
Cellular Componentmembrane
Cellular Componentmicrotubule
Cellular Componentspindle
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular Functionisomerase activity
Molecular Functionmicrotubule binding
Molecular Functionmicrotubule severing ATPase activity
Biological Processmicrotubule severing
Biological Processpositive regulation of microtubule depolymerization
Biological Processprotein hexamerization

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Spastin
  • EC number

Gene names

    • ORF names
      HICCMSTLAB_LOCUS12685

Organism names

Accessions

  • Primary accession
    A0A8J2HQJ7

Proteomes

Subcellular Location

Endomembrane system
Membrane
; Peripheral membrane protein
Cytoplasm, cytoskeleton
Note: Forms an intramembrane hairpin-like structure in the membrane.

Features

Showing features for topological domain, transmembrane, intramembrane.

Type
IDPosition(s)Description
Topological domain1-74Cytoplasmic
Transmembrane68-97Helical
Intramembrane75-95Helical
Topological domain96-722Cytoplasmic

Keywords

Interaction

Subunit

Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules.

Family & Domains

Features

Showing features for region, compositional bias, domain.

Type
IDPosition(s)Description
Region1-31Disordered
Region36-55Disordered
Compositional bias38-54Basic residues
Domain159-237MIT
Region254-281Disordered
Region332-422Disordered
Compositional bias350-393Polar residues
Compositional bias403-417Polar residues
Domain480-616AAA+ ATPase

Sequence similarities

Belongs to the AAA ATPase family. Spastin subfamily.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    722
  • Mass (Da)
    80,389
  • Last updated
    2022-05-25 v1
  • Checksum
    B85E6227C4132857
MSYSDGGRSVRKSGSKSPKKLRVTKLENSDKATTTISYGSHHHHHHHHHHHRHNLIDAPQPSIHKRNLYIVSFPLILVFNVLRTLLYQLFVVFKYLYASTNHLIRRTNTNKSQCQFEIIVRPETNDLLAVSSDQDNSVMSQIARRPAGPGPGDPLLAKQKHHHRRAFEFISKALKIDEENEGHKEMAIELYKKGIGELEKGVAVECYGGRGEVYERAQRLHEKMRANLVMAKDRLDFLANICALKKLELANDCRGNEKSTQESASDTSDSYDKIKYPRNRKNNMILRSNESLTGPSNNTIKRCVNNSNTANAHTHINKSTIPKPISPKISHIRTSEASGRKLTVPGKRVPGTISKSQTLPRSMGRSTPTHSCHRTTPIKPSGTPPSIKRQLSVPGNGSPIRRPGTPGGSNSNRGTPSRKVPMLKGVDPKLAQVILDEILEGGTAVQWNDIAGQEVAKQALQEMVILPSLRPELFTGLRTPARGLLLFGPPGNGKTLLARAVATQCNATFFSISAASLTSKYVGEGEKLVRALFAIARELQPSVIFIDEVDSLLSERRDNEHEASRRLKTEFLVEFDGLPCNPDERVLVMAATNRPQELDEAALRRFPKRVYVTLPDLQTRVTLLKRLLGKHSDPLTPEELYKTAVLTDGYSGSDLTGLAKDAALGPIRELNPEQVKALDLNLVRNITMQDFLDSLKRIRRSVSPSSLTAFEKWSFEYGDVSL

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias38-54Basic residues
Compositional bias350-393Polar residues
Compositional bias403-417Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CAJNRD030001124
EMBL· GenBank· DDBJ
CAG5107310.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp