A0A8J2FNZ1 · A0A8J2FNZ1_9BACT
- ProteinPhosphoenolpyruvate-protein phosphotransferase
- GeneptsI
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids610 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).
Catalytic activity
- L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-L-histidyl-[protein] + pyruvate
Cofactor
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 203 | Tele-phosphohistidine intermediate | |||
Binding site | 310 | phosphoenolpyruvate (UniProtKB | ChEBI) | |||
Binding site | 346 | phosphoenolpyruvate (UniProtKB | ChEBI) | |||
Binding site | 446 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 469-470 | phosphoenolpyruvate (UniProtKB | ChEBI) | |||
Binding site | 470 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 480 | phosphoenolpyruvate (UniProtKB | ChEBI) | |||
Active site | 517 | Proton donor | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | kinase activity | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoenolpyruvate-protein phosphotransferase activity | |
Biological Process | phosphoenolpyruvate-dependent sugar phosphotransferase system | |
Biological Process | phosphorylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoenolpyruvate-protein phosphotransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Verrucomicrobiota > Methylacidiphilae > Methylacidiphilales > Methylacidiphilaceae > Candidatus Methylacidithermus
Accessions
- Primary accessionA0A8J2FNZ1
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain, coiled coil.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 17-140 | Phosphotransferase system enzyme I N-terminal | |||
Coiled coil | 54-81 | ||||
Domain | 167-239 | PEP-utilising enzyme mobile | |||
Domain | 266-556 | PEP-utilising enzyme C-terminal | |||
Sequence similarities
Belongs to the PEP-utilizing enzyme family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length610
- Mass (Da)68,247
- Last updated2022-05-25 v1
- MD5 ChecksumE04FF9B60A29DF45363396D1A4A4A772
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CAJNOB010000023 EMBL· GenBank· DDBJ | CAF0698948.1 EMBL· GenBank· DDBJ | Genomic DNA |