A0A8J2BRD1 · A0A8J2BRD1_9BACT

Function

function

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site208-211CoA (UniProtKB | ChEBI)
Binding site326CoA (UniProtKB | ChEBI)
Binding site402-404ATP (UniProtKB | ChEBI)
Binding site426-431ATP (UniProtKB | ChEBI)
Binding site515ATP (UniProtKB | ChEBI)
Binding site530ATP (UniProtKB | ChEBI)
Binding site538CoA (UniProtKB | ChEBI)
Binding site541ATP (UniProtKB | ChEBI)
Binding site552Mg2+ (UniProtKB | ChEBI)
Binding site554Mg2+ (UniProtKB | ChEBI)
Binding site557Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionacetate-CoA ligase activity
Molecular FunctionAMP binding
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Biological Processacetyl-CoA biosynthetic process from acetate

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Acetyl-coenzyme A synthetase
  • EC number
  • Short names
    AcCoA synthetase
    ; Acs
  • Alternative names
    • Acetate--CoA ligase
    • Acyl-activating enzyme

Gene names

    • Name
      acs
    • Synonyms
      acsA
    • ORF names
      MPNT_70034

Organism names

Accessions

  • Primary accession
    A0A8J2BRD1

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue624N6-acetyllysine

Post-translational modification

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain44-97Acetyl-coenzyme A synthetase N-terminal
Domain109-485AMP-dependent synthetase/ligase
Domain546-624AMP-binding enzyme C-terminal

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    674
  • Mass (Da)
    75,824
  • Last updated
    2022-05-25 v1
  • Checksum
    1A0988DD05C6AE3E
MRNSDEKSPDEVRGITAGLTKIRNIPSPRWRGTRKPRISSLTQYRRLYRESLQKPEVFWAEQARKISWYRKWRTVSQWEPPFARWFVGGELNACVNCVDRHLEGPRRYKAAIVWEGEPQGQIRVLTYQELHREVCRLANVLLRHGVRRGDRVVIYLPMIPEALITMLACARIGAVHSVVFGGFGPGALQERILDSQARLVVTADGGYRRGAIIPLKENLDAALAELSCVQTVLVVRRTHCPIPWVEGRDYWLDQELEKVRSFHTAERMGSEDPLFILYTSGSTGKPKGVLHTTAGYLLGAMVTTEYIFDLRDEDLYWCTADVGWITGHSYVVYGPLACGGTVFVYEGAPDHPSPSRFWEMIERHRISIFYTAPTAIRAFLRWGEEWLSKSDLSSLRLLGSVGEPINPEVWMWYFEKVGGGRCPVVDTWWQTETGAIMIAPLPGVTPLKPGSATLPFFGVDAAVVDPEGNELPPGNKGRLVLRRPWPSMLRTLYQDPDAYRQIYWTPVPGCYFTGDAAYRDQDGYFWIVGRMDDVLNVSGHRLGTAEVESALVSHPAVAEAAVVGRPDPLKGQALVAFVILKEKASPTQALMEELRAHVARQIGPIAKPEEIHFTEALPKTRSGKIMRRLLKSVANRSEVRGDLSTLEDQTVLQKLAAARSEPIIGEAEPESWED

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CAJNOB010000067
EMBL· GenBank· DDBJ
CAF0704474.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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