A0A8I6GKR7 · A0A8I6GKR7_RAT
- ProteinUbiquitin conjugation factor E4
- GeneUbe4b
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1305 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. Also functions as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates.
Catalytic activity
Pathway
Protein modification; protein ubiquitination.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Cellular Component | ubiquitin ligase complex | |
Molecular Function | ATP binding | |
Molecular Function | enzyme binding | |
Molecular Function | ubiquitin protein ligase activity | |
Molecular Function | ubiquitin-ubiquitin ligase activity | |
Biological Process | ERAD pathway | |
Biological Process | granzyme-mediated apoptotic signaling pathway | |
Biological Process | neuron projection development | |
Biological Process | proteasome-mediated ubiquitin-dependent protein catabolic process | |
Biological Process | protein autoubiquitination | |
Biological Process | protein catabolic process | |
Biological Process | protein monoubiquitination | |
Biological Process | protein polyubiquitination | |
Biological Process | protein ubiquitination | |
Biological Process | response to endoplasmic reticulum stress | |
Biological Process | response to UV | |
Biological Process | ubiquitin-dependent protein catabolic process | |
Biological Process | ventricular trabecula myocardium morphogenesis |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUbiquitin conjugation factor E4
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionA0A8I6GKR7
Proteomes
Organism-specific databases
Subcellular Location
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-15 | Basic and acidic residues | ||||
Sequence: MEELSADEIRRRRLA | ||||||
Region | 1-155 | Disordered | ||||
Sequence: MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASVPGPSQSLGLNVHNTTPATSPIGAAGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGPEVSE | ||||||
Compositional bias | 31-45 | Pro residues | ||||
Sequence: SPQRENPPGPPIAAS | ||||||
Compositional bias | 49-66 | Polar residues | ||||
Sequence: PSQSLGLNVHNTTPATSP | ||||||
Compositional bias | 76-123 | Polar residues | ||||
Sequence: SQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVD | ||||||
Compositional bias | 129-151 | Basic and acidic residues | ||||
Sequence: MEVDENDRREKRSLSDKEPSSGP | ||||||
Region | 296-328 | Disordered | ||||
Sequence: PSHTAAAVPSPSLSSHSTASVIATGSQPSSPRY | ||||||
Region | 348-410 | Disordered | ||||
Sequence: SILANPPGFPAHSSSPRAVPASSSRQRPRSRAPAFPPASPSAASRRPSSLRISPSLGASGGAS | ||||||
Compositional bias | 389-410 | Polar residues | ||||
Sequence: AASRRPSSLRISPSLGASGGAS | ||||||
Region | 1061-1080 | Disordered | ||||
Sequence: QEQWDQLPRDQQQARQSQLA | ||||||
Compositional bias | 1065-1080 | Polar residues | ||||
Sequence: DQLPRDQQQARQSQLA | ||||||
Domain | 1230-1303 | U-box | ||||
Sequence: DAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQMLTESMLEPVPELKEQIQAWMREKQSS |
Domain
The U-box domain is required for the ubiquitin protein ligase activity.
Sequence similarities
Belongs to the ubiquitin conjugation factor E4 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,305
- Mass (Da)146,898
- Last updated2022-05-25 v1
- ChecksumAA611F6F831C45A0
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F1M8V2 | F1M8V2_RAT | Ube4b | 1173 | ||
A0A8I6GAW9 | A0A8I6GAW9_RAT | Ube4b | 1351 | ||
A0A8I6G4J1 | A0A8I6G4J1_RAT | Ube4b | 1100 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-15 | Basic and acidic residues | ||||
Sequence: MEELSADEIRRRRLA | ||||||
Compositional bias | 31-45 | Pro residues | ||||
Sequence: SPQRENPPGPPIAAS | ||||||
Compositional bias | 49-66 | Polar residues | ||||
Sequence: PSQSLGLNVHNTTPATSP | ||||||
Compositional bias | 76-123 | Polar residues | ||||
Sequence: SQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVD | ||||||
Compositional bias | 129-151 | Basic and acidic residues | ||||
Sequence: MEVDENDRREKRSLSDKEPSSGP | ||||||
Compositional bias | 389-410 | Polar residues | ||||
Sequence: AASRRPSSLRISPSLGASGGAS | ||||||
Compositional bias | 1065-1080 | Polar residues | ||||
Sequence: DQLPRDQQQARQSQLA |
Keywords
- Technical term