A0A8I6AD25 · A0A8I6AD25_RAT

Function

function

Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

  • (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate = 12-oxo-(5Z,8Z,10E,14Z)-eicosatetraenoate + H2O
    This reaction proceeds in the forward direction.
    EC:4.2.1.152 (UniProtKB | ENZYME | Rhea)
  • (13S)-hydroperoxy-(9Z,11E)-octadecadienoate = 13-oxo-(9Z,11E)-octadecadienoate + H2O
    This reaction proceeds in the forward direction.
  • (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate = 15-oxo-(5Z,8Z,11Z,13E)-eicosatetraenoate + H2O
    This reaction proceeds in the forward direction.
  • (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 + reduced [NADPH--hemoprotein reductase] = (19R,20S)-epoxy-(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate = 5-oxo-(6E,8Z,11Z,14Z)-eicosatetraenoate + H2O
    This reaction proceeds in the forward direction.
  • (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 + reduced [NADPH--hemoprotein reductase] = (14R,15S)-epoxy-(5Z,8Z,11Z)-eicosatrienoate + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 + reduced [NADPH--hemoprotein reductase] = 19-hydroxy-(5Z,8Z,11Z,14Z)-eicosatetraenoate + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + O2 + reduced [NADPH--hemoprotein reductase] = (17R,18S)-epoxy-(5Z,8Z,11Z,14Z)-eicosatetraenoate + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • 17beta-estradiol + O2 + reduced [NADPH--hemoprotein reductase] = 2-hydroxy-17beta-estradiol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • 17beta-estradiol + O2 + reduced [NADPH--hemoprotein reductase] = 4-hydroxy-17beta-estradiol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • all-trans-retinal + O2 + reduced [NADPH--hemoprotein reductase] = all-trans-retinoate + 2 H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • all-trans-retinol + O2 + reduced [NADPH--hemoprotein reductase] = all-trans-retinal + H+ + 2 H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • estrone + O2 + reduced [NADPH--hemoprotein reductase] = 2-hydroxyestrone + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • estrone + O2 + reduced [NADPH--hemoprotein reductase] = 4-hydroxyestrone + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Pathway

Cofactor metabolism; retinol metabolism.
Lipid metabolism.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site476Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Molecular Functionaromatase activity
Molecular Functioncaffeine oxidase activity
Molecular Functiondemethylase activity
Molecular Functionenzyme binding
Molecular Functionestrogen 16-alpha-hydroxylase activity
Molecular Functionestrogen 2-hydroxylase activity
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionlyase activity
Biological Processalkaloid metabolic process
Biological Processcellular respiration
Biological Processcellular response to cadmium ion
Biological Processdibenzo-p-dioxin metabolic process
Biological Processestrogen metabolic process
Biological Processhydrogen peroxide biosynthetic process
Biological Processlung development
Biological Processmonocarboxylic acid metabolic process
Biological Processmonoterpenoid metabolic process
Biological Processoxidative demethylation
Biological Processporphyrin-containing compound metabolic process
Biological Processpost-embryonic development
Biological Processregulation of gene expression
Biological Processretinol metabolic process
Biological Processsteroid catabolic process
Biological Processtoxin biosynthetic process
Biological Processxenobiotic catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytochrome P450 1A
  • EC number

Gene names

    • Name
      Cyp1a2

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Brown Norway
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    A0A8I6AD25

Proteomes

Organism-specific databases

Subcellular Location

Endoplasmic reticulum membrane
; Peripheral membrane protein
Microsome membrane
; Peripheral membrane protein
Membrane
; Peripheral membrane protein

Keywords

Structure

3D structure databases

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    533
  • Mass (Da)
    60,365
  • Last updated
    2022-05-25 v1
  • Checksum
    BCB8C52BDE4E63B5
MEGSESIFNQSISISPAVVQMAFSQYISLAPELLLATAIFCLVFWVLRGTRTQVPKGLKSPPGPWGLPFIGHMLTLGKNPHLSLTKLSQQYGDVLQIRIGSTPVVVLSGLNTIKQALVKQGDDFKGRPDLYSFTLITNGKSMTFNPDSGPVWAARRRLAQDALKSFSIASDPTSVSSCYLEEHVSKEANHLISKFQKLMAEVGHFEPVNQVVESVANVIGAMCFGKNFPRKSEEMLNLVKSSKDFVENVTSGNAVDFFPVLRYLPNPALKRFKNFNDNFVLFLQKTVQEHYQDFNKNSIQDITGALFKHSENYKDNGGLIPQEKIVNIVNDIFGAGFETVTTAIFWSILLLVTEPKVQRKIHEELDTVIGRDRQPRLSDRPQLPYLEAFILEIYRYTSFVPFTIPHSTTRDTSLNGFHIPKECCIFINQWQVNHDEKQWKDPFVFRPERFLTNDNTAIDKTLSEKVMLFGLGKRRCIGEIPAKWEVFLFLAILLHQLEFTVPPGVKVDLTPSYGLTMKPRTCEHVQAWPRFSK

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
P04799CP1A2_RATCyp1a2513

Keywords

Genome annotation databases

Similar Proteins

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