A0A8I5Y8D9 · A0A8I5Y8D9_RAT
- ProteinMedium-chain specific acyl-CoA dehydrogenase, mitochondrial
- GeneAcadm
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids412 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Electron transfer flavoprotein (ETF) is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 6 to 12 carbons long primary chains.
Catalytic activity
- oxidized [electron-transfer flavoprotein] + hexadecanoyl-CoA + H+ = (2E)-hexadecenoyl-CoA + reduced [electron-transfer flavoprotein]This reaction proceeds in the forward direction.
- hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]This reaction proceeds in the forward direction.
- octanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-octenoyl-CoA + reduced [electron-transfer flavoprotein]This reaction proceeds in the forward direction.
- pentanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-pentenoyl-CoA + reduced [electron-transfer flavoprotein]This reaction proceeds in the forward direction.
- tetradecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-tetradecenoyl-CoA + reduced [electron-transfer flavoprotein]This reaction proceeds in the forward direction.
- decanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-decenoyl-CoA + reduced [electron-transfer flavoprotein]This reaction proceeds in the forward direction.
- dodecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-dodecenoyl-CoA + reduced [electron-transfer flavoprotein]This reaction proceeds in the forward direction.
Cofactor
Pathway
Lipid metabolism; mitochondrial fatty acid beta-oxidation.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 149-158 | FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 182-184 | FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 297-299 | FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 307-308 | FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 365-369 | FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Active site | 392 | Proton acceptor | |||
Binding site | 394-396 | FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial matrix | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | medium-chain fatty acyl-CoA dehydrogenase activity | |
Biological Process | fatty acid beta-oxidation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMedium-chain specific acyl-CoA dehydrogenase, mitochondrial
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionA0A8I5Y8D9
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 33-142 | Acyl-CoA dehydrogenase/oxidase N-terminal | |||
Domain | 148-246 | Acyl-CoA oxidase/dehydrogenase middle | |||
Domain | 258-405 | Acyl-CoA dehydrogenase/oxidase C-terminal | |||
Sequence similarities
Belongs to the acyl-CoA dehydrogenase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length412
- Mass (Da)45,568
- Last updated2022-05-25 v1
- MD5 Checksum63AA39C0EF4D46AA4DE806815AD60EDF
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
P08503 | ACADM_RAT | Acadm | 421 | ||
G3V796 | G3V796_RAT | Acadm | 421 | ||
A0A8I5ZQ05 | A0A8I5ZQ05_RAT | Acadm | 454 |
Keywords
- Technical term