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A0A8I5Y8D9 · A0A8I5Y8D9_RAT

  • Protein
    Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
  • Gene
    Acadm
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Electron transfer flavoprotein (ETF) is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 6 to 12 carbons long primary chains.

Catalytic activity

  • oxidized [electron-transfer flavoprotein] + hexadecanoyl-CoA + H+ = (2E)-hexadecenoyl-CoA + reduced [electron-transfer flavoprotein]
    This reaction proceeds in the forward direction.
  • hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]
    This reaction proceeds in the forward direction.
  • octanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-octenoyl-CoA + reduced [electron-transfer flavoprotein]
    This reaction proceeds in the forward direction.
  • pentanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-pentenoyl-CoA + reduced [electron-transfer flavoprotein]
    This reaction proceeds in the forward direction.
  • tetradecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-tetradecenoyl-CoA + reduced [electron-transfer flavoprotein]
    This reaction proceeds in the forward direction.
  • a medium-chain 2,3-saturated fatty acyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = a medium-chain (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]
    This reaction proceeds in the forward direction.
    EC:1.3.8.7 (UniProtKB | ENZYME | Rhea)
  • decanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-decenoyl-CoA + reduced [electron-transfer flavoprotein]
    This reaction proceeds in the forward direction.
  • dodecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-dodecenoyl-CoA + reduced [electron-transfer flavoprotein]
    This reaction proceeds in the forward direction.

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Pathway

Lipid metabolism; mitochondrial fatty acid beta-oxidation.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site149-158FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site182-184FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site297-299FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site307-308FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site365-369FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Active site392Proton acceptor
Binding site394-396FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain

GO annotations

AspectTerm
Cellular Componentmitochondrial matrix
Molecular Functionflavin adenine dinucleotide binding
Molecular Functionmedium-chain fatty acyl-CoA dehydrogenase activity
Biological Processfatty acid beta-oxidation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
  • EC number

Gene names

    • Name
      Acadm

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Brown Norway
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    A0A8I5Y8D9

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain33-142Acyl-CoA dehydrogenase/oxidase N-terminal
Domain148-246Acyl-CoA oxidase/dehydrogenase middle
Domain258-405Acyl-CoA dehydrogenase/oxidase C-terminal

Sequence similarities

Belongs to the acyl-CoA dehydrogenase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    412
  • Mass (Da)
    45,568
  • Last updated
    2022-05-25 v1
  • MD5 Checksum
    63AA39C0EF4D46AA4DE806815AD60EDF
DVLRSVSHFECRAQHTKPSLKQEPGLGFSFELTEQQKEFQTIARKFAREEIIPVAPDYDKSGEYPFPLIKRAWELGLINTHIPESCGGLGLGTFDACLITEELAYGCTGVQTAIEANSLGQMPVIIAGNDQQKKKYLGRMTEQLMMCAYCVTEPSAGSDVAGIKTKAEKKGDEYVINGQKMWITNGGKANWYFVLTRSNPDPKVPASKAFTGFIVEADTPGIHIGKKELNMGQRCSDTRGITFEDVRVPKENVLIGEGAGFKIAMGAFDRTRPTVAAGAVGLAQRALDEATKYALDRKTFGKLLVEHQGVSFLLAEMAMKVELARLSYQRAAWEVDSGRRNTYFASIAKAFAGDIANQLATDAVQIFGGYGFNTEYPVEKLMRDAKIYQIYEGTAQIQRLIIAREHIEKYKN

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
P08503ACADM_RATAcadm421
G3V796G3V796_RATAcadm421
A0A8I5ZQ05A0A8I5ZQ05_RATAcadm454

Keywords

Genome annotation databases

Similar Proteins

Disclaimer

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