A0A8I5KTS5 · A0A8I5KTS5_HUMAN
- ProteinProtein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase
- GenePOMGNT1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids527 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Participates in O-mannosyl glycosylation by catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties. Is specific for alpha linked terminal mannose.
Catalytic activity
- 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl)-L-threonyl-[protein] + UDP + H+
Cofactor
Note: The manganese ion interacts primarily with the substrate UDP-N-acetylglucosamine.
Pathway
Protein modification; protein glycosylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Golgi membrane | |
Molecular Function | acetylglucosaminyltransferase activity | |
Molecular Function | carbohydrate binding | |
Molecular Function | manganese ion binding | |
Biological Process | protein glycosylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase
- EC number
- Short namesPOMGnT1
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionA0A8I5KTS5
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Single-pass type II membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for modified residue (large scale data).
Type | ID | Position(s) | Source | Description | ||
---|---|---|---|---|---|---|
Modified residue (large scale data) | 7 | PRIDE | Phosphoserine | |||
Modified residue (large scale data) | 66 | PRIDE | Phosphoserine | |||
Modified residue (large scale data) | 78 | PRIDE | Phosphotyrosine | |||
Keywords
- PTM
Proteomic databases
Interaction
Subunit
Interacts with DAG1 (via O-linked mannose moiety). Interacts (via transmembrane domain) with FKTN; the interaction is direct and is required for normal location in Golgi membranes.
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 68-96 | Disordered | |||
Compositional bias | 78-96 | Basic and acidic residues | |||
Domain | 129-218 | ILEI/PANDER | |||
Domain
The stem domain mediates specific interaction with beta-linked N-acetylglucosamine moieties of O-glycosylated proteins. It also interacts with its product, N-acetyl-beta-D-glucosaminyl-(1->2)-O-alpha-D-mannosylprotein.
Sequence similarities
Belongs to the glycosyltransferase 13 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length527
- Mass (Da)60,047
- Last updated2022-05-25 v1
- Checksum9E3B7F2395D1A909
Computationally mapped potential isoform sequences
There are 10 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q8WZA1 | PMGT1_HUMAN | POMGNT1 | 660 | ||
A0A8I5QJA9 | A0A8I5QJA9_HUMAN | POMGNT1 | 317 | ||
A0A8I5QJV3 | A0A8I5QJV3_HUMAN | POMGNT1 | 546 | ||
Q68CV6 | Q68CV6_HUMAN | POMGNT1 | 200 | ||
A0A8I5KNB7 | A0A8I5KNB7_HUMAN | POMGNT1 | 718 | ||
A0A8I5KPT5 | A0A8I5KPT5_HUMAN | POMGNT1 | 82 | ||
A0A8I5KQE4 | A0A8I5KQE4_HUMAN | POMGNT1 | 327 | ||
A0A8I5KR25 | A0A8I5KR25_HUMAN | POMGNT1 | 627 | ||
A0A8I5KSR6 | A0A8I5KSR6_HUMAN | POMGNT1 | 47 | ||
A0A8I5KVA5 | A0A8I5KVA5_HUMAN | POMGNT1 | 673 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 78-96 | Basic and acidic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL672043 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |