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A0A8H4S0N4 · A0A8H4S0N4_9EURO

Function

function

NADH-dependent reductase for DPH3 and cytochrome b5. Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase, predominantly CBR1. By reducing DPH3, also involved in the formation of the tRNA wobble base modification mcm5s 2U (5-methoxycarbonylmethyl-2-thiouridine), mediated by the elongator complex. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Pathway

Protein modification; peptidyl-diphthamide biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site109FAD (UniProtKB | ChEBI)
Binding site111FAD (UniProtKB | ChEBI)
Binding site126FAD (UniProtKB | ChEBI)
Binding site128FAD (UniProtKB | ChEBI)
Binding site135FAD (UniProtKB | ChEBI)
Binding site136FAD (UniProtKB | ChEBI)
Binding site177FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum
Cellular Componentmitochondrial outer membrane
Molecular Functionoxidoreductase activity
Molecular Functiontransferase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADH-cytochrome b5 reductase
  • EC number

Gene names

    • ORF names
      HAV15_007772

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • #12
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Penicillium

Accessions

  • Primary accession
    A0A8H4S0N4

Proteomes

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane28-44Helical

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain55-160FAD-binding FR-type

Sequence similarities

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    304
  • Mass (Da)
    33,158
  • Last updated
    2022-01-19 v1
  • MD5 Checksum
    A64B8CC5159A3D3E83C696AC49E6D82E
MSLATIGTALAPSALLIASVYAIKPEFIAYAIAVAGILAGYLVFSNKPRKVLNPTEFQNFVLKEKNEISHNVAIYRFALPRSTDILGLPIGQHISLAATIAGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKYLTELKIGDNMKVRGPKGAMVYTPNMCRHIGMISGGTGITPMLQVIKAIIRNRPRNGGNDTTKVDLIFANVNPEDILLKDQLEELEKEDDGFRVYYVLNNPPEGWTGGVGFVTPDMIKERLPAPAADVKILLCGPPPMVSAMKKSTEALGYTKARPVSKLEDQVFCF

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAASRZ010000051
EMBL· GenBank· DDBJ
KAF4760156.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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