A0A8H4S0N4 · A0A8H4S0N4_9EURO
- ProteinNADH-cytochrome b5 reductase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids304 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
NADH-dependent reductase for DPH3 and cytochrome b5. Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase, predominantly CBR1. By reducing DPH3, also involved in the formation of the tRNA wobble base modification mcm5s 2U (5-methoxycarbonylmethyl-2-thiouridine), mediated by the elongator complex. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes.
Catalytic activity
- 2 Fe3+-[Dph3] + NADH = 2 Fe2+-[Dph3] + NAD+ + H+This reaction proceeds in the forward direction.
Cofactor
Pathway
Protein modification; peptidyl-diphthamide biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 109 | FAD (UniProtKB | ChEBI) | |||
Binding site | 111 | FAD (UniProtKB | ChEBI) | |||
Binding site | 126 | FAD (UniProtKB | ChEBI) | |||
Binding site | 128 | FAD (UniProtKB | ChEBI) | |||
Binding site | 135 | FAD (UniProtKB | ChEBI) | |||
Binding site | 136 | FAD (UniProtKB | ChEBI) | |||
Binding site | 177 | FAD (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | mitochondrial outer membrane | |
Molecular Function | oxidoreductase activity | |
Molecular Function | transferase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADH-cytochrome b5 reductase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Penicillium
Accessions
- Primary accessionA0A8H4S0N4
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Mitochondrion outer membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 28-44 | Helical | |||
Keywords
- Cellular component
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length304
- Mass (Da)33,158
- Last updated2022-01-19 v1
- MD5 ChecksumA64B8CC5159A3D3E83C696AC49E6D82E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JAASRZ010000051 EMBL· GenBank· DDBJ | KAF4760156.1 EMBL· GenBank· DDBJ | Genomic DNA |