A0A8H3XW86 · A0A8H3XW86_ASPTU
- Proteinalpha-1,2-Mannosidase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids513 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.
Catalytic activity
- N4-(alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N4-(alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose
- N4-(alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N4-(alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose
Cofactor
Protein has several cofactor binding sites:
Pathway
Protein modification; protein glycosylation.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 124 | Proton donor | |||
Active site | 269 | ||||
Active site | 377 | Proton donor | |||
Active site | 411 | ||||
Binding site | 503 | Ca2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasmic vesicle lumen | |
Cellular Component | endoplasmic reticulum | |
Cellular Component | membrane | |
Molecular Function | calcium ion binding | |
Molecular Function | mannosyl-oligosaccharide 1,2-alpha-mannosidase activity | |
Biological Process | carbohydrate metabolic process | |
Biological Process | mannose trimming involved in glycoprotein ERAD pathway |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namealpha-1,2-Mannosidase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Circumdati
Accessions
- Primary accessionA0A8H3XW86
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for disulfide bond.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Disulfide bond | 334↔363 | ||||
Keywords
- PTM
Interaction
Subunit
Monomer.
Sequence
- Sequence statusComplete
- Length513
- Mass (Da)55,875
- Last updated2022-01-19 v1
- Checksum0FDAB2CB27E93724
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BRPE01000020 EMBL· GenBank· DDBJ | GLA89929.1 EMBL· GenBank· DDBJ | Genomic DNA |