A0A8F9RA17 · A0A8F9RA17_HRSV
- ProteinRNA-directed RNA polymerase L
- GeneL
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids2165 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N7-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular protein N concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals.
Catalytic activity
- GTP + H2O = GDP + H+ + phosphate
- a 5'-end (5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-homocysteine
- a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + H+ + S-adenosyl-L-homocysteine
Cofactor
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | host cell cytoplasm | |
Cellular Component | virion component | |
Molecular Function | ATP binding | |
Molecular Function | hydrolase activity | |
Molecular Function | mRNA 5'-cap (guanine-N7-)-methyltransferase activity | |
Molecular Function | RNA-dependent RNA polymerase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameRNA-directed RNA polymerase L
- Short namesProtein L
- Alternative names
Including 5 domains:
- Recommended nameRNA-directed RNA polymerase
- EC number
- Recommended nameGTP phosphohydrolase
- EC number
- Recommended nameGDP polyribonucleotidyltransferase
- EC number
- Alternative names
- Recommended namemRNA (nucleoside-2'-O-)-methyltransferase
- EC number
- Short namesN1-2'-O-MTase
- Recommended namemRNA (guanine-N(7)-)-methyltransferase
- Short namesG-N7-MTase
Gene names
Organism names
- Strains
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Negarnaviricota > Haploviricotina > Monjiviricetes > Mononegavirales > Pneumoviridae > Orthopneumovirus > Orthopneumovirus hominis
Accessions
- Primary accessionA0A8F9RA17
Subcellular Location
Interaction
Subunit
Interacts with the phosphoprotein (via C-terminus); the association of P and L forms the polymerase complex.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 693-877 | RdRp catalytic | ||||
Sequence: SKCSIITDLSKFNQAFRYETSCICSDVLDELHGVQSLFSWLHLTIPHVTIICTYRHAPPYIKDHIVDLNNVDEQSGLYRYHMGGIEGWCQKLWTIEAISLLDLISLKGKFSITALINGDNQSIDISKPVRLMEGQTHAQADYLLALNSLKLLYKEYAGIGHKLKGTETYISRDMQFMSKTIQHNG | ||||||
Domain | 1820-2008 | Mononegavirus-type SAM-dependent 2'-O-MTase | ||||
Sequence: RFNFVFSSTGCKISIEYILKDLKIKDPNCIAFIGEGAGNLLLRTVVELHPDIRYIYRSLKDCNDHSLPIEFLRLYNGHINIDYGENLTIPATDATNNIHWSYLHIKFAEPISLFVCDAELPVTVNWSKIIIEWSKHVRKCKYCSSVNKCTLIVKYHAQDDIDFKLDNITILKTYVCLGSKLKGSEVYLV |
Sequence similarities
Belongs to the paramyxovirus L protein family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length2,165
- Mass (Da)250,227
- Last updated2022-01-19 v1
- ChecksumF0BAE98F8012E3AD