A0A8F9RA17 · A0A8F9RA17_HRSV

Function

function

Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N7-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular protein N concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals.

Catalytic activity

  • GTP + H2O = GDP + H+ + phosphate
  • a 5'-end (5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-homocysteine
  • a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + 2 S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + H+ + 2 S-adenosyl-L-homocysteine
    EC:2.1.1.375 (UniProtKB | ENZYME | Rhea)
  • a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + H+ + S-adenosyl-L-homocysteine
  • a 5'-end triphospho-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + GDP + H+ = a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + diphosphate
    EC:2.7.7.88 (UniProtKB | ENZYME | Rhea)
  • a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
    EC:2.7.7.48 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componenthost cell cytoplasm
Cellular Componentvirion component
Molecular FunctionATP binding
Molecular Functionhydrolase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular FunctionRNA-dependent RNA polymerase activity

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    RNA-directed RNA polymerase L
  • Short names
    Protein L
  • Alternative names
    • Large structural protein
    • Replicase
    • Transcriptase

Including 5 domains:

  • Recommended name
    RNA-directed RNA polymerase
  • EC number
  • Recommended name
    GTP phosphohydrolase
  • EC number
  • Recommended name
    GDP polyribonucleotidyltransferase
  • EC number
  • Alternative names
    • PRNTase
  • Recommended name
    mRNA (nucleoside-2'-O-)-methyltransferase
  • EC number
  • Short names
    N1-2'-O-MTase
  • Recommended name
    mRNA (guanine-N(7)-)-methyltransferase
  • Short names
    G-N7-MTase

Gene names

    • Name
      L

Organism names

  • Taxonomic identifier
  • Strains
    • RSVA/Novosibirsk/7.63Hl/2020
    • RSVA/Novosibirsk/8.46Hl/2020
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Negarnaviricota > Haploviricotina > Monjiviricetes > Mononegavirales > Pneumoviridae > Orthopneumovirus > Orthopneumovirus hominis

Accessions

  • Primary accession
    A0A8F9RA17

Subcellular Location

Keywords

Interaction

Subunit

Interacts with the phosphoprotein (via C-terminus); the association of P and L forms the polymerase complex.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain693-877RdRp catalytic
Domain1820-2008Mononegavirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the paramyxovirus L protein family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,165
  • Mass (Da)
    250,227
  • Last updated
    2022-01-19 v1
  • Checksum
    F0BAE98F8012E3AD
MDPIISGNSANVYLTDSYLKGVISFSECNALGSYIFNGPYLKNDYTNLISRQNPLIEHINLKKLNITQSLISKYHKGEIKMEEPTYFQSLLMTYKSMTTSEQTTTTNLLKKIIRRAIEISDVKVYAILNKLGLKEKDKIKSNDGQDEDNSVITTIIKDDILLAVKDNQSHLKADKNQSSKQKDTIKTTLLKKLMCSMQHPPSWLIHWFNLYTKLNSILTQYRSSEVKNHGFILIDNHTLSGFQFILNQYGCIVYHKELKRITVTTYNQFLTWKDISLSRLNVCLITWISNCLNTLNKSLGLRCGFNNVILTQLFLYGDCILKLFHNEGFYIIKEVEGFIMSLILNITEEDQFRKRFYNSMLNNITDAANKAQKNLLSRVCHTLLDKTISDNIINGRWIILLSKFLKLIKLAGDNNLNNLSELYFLFRIFGHPMVDERQAMDAVKVNCNETKFYLLSSLSMLRGAFIYRIIKGFVNNYNRWPTLRNAIVLPLRWLTYYKLNTYPSLLELTERDLIVLSGLRFYREFRLPKKVDLEMIINDKAISPPKNLIWTSFPRNYMPSHIQNYIEHEKLKFSDSDKSRRVLEYYLRDNKFNECDLHNCVVNQSYLNNPNHVVSLTGKERELSVGRMFAMQPGMFRQVQILAEKMIAENILQFFPESLTRYGDLELQKILELKAGISNKSNRYNDNYNNYISKCSIITDLSKFNQAFRYETSCICSDVLDELHGVQSLFSWLHLTIPHVTIICTYRHAPPYIKDHIVDLNNVDEQSGLYRYHMGGIEGWCQKLWTIEAISLLDLISLKGKFSITALINGDNQSIDISKPVRLMEGQTHAQADYLLALNSLKLLYKEYAGIGHKLKGTETYISRDMQFMSKTIQHNGVYYPASIKKVLRVGPWINTILDDFKVSLESIGSLTQELEYRGESLLCSLIFRNVWLYNQIALQLKNHALCNNKLYLDILKVLKHLKTFFNLDNIDTALTLYMNLPMLFGGGDPNLLYRSFYRRTPDFLTEAIVHSVFILSYYTNHDLKDKLQDLSDDRLNKFLTCIITFDKNPNAEFVTLMRDPQALGSERQAKITSEINRLAVTEVLSTAPNKIFSKSAQHYTTTEIDLNDIMQNIEPTYPHGLRVVYESLPFYKAEKIVNLISGTKSITNILEKTSAIDLTDIDRATEMMRKNITLLIRILPLDFNRDKREILSMENLSITELSKYVRERSWSLSNIVGVTSPSIMYTMDIKYTTSTIASGIIIEKYNVNSLTRGERGPTKPWVGSSTQEKKTMPVYNRQVLTKKQRDQIDLLAKLDWVYASIDNKDEFMEELSIGTLGLTYEKAKKLFPQYLSVNYLHRLTVSSRPCEFPASIPAYRTTNYHFDTSPINRILTEKYGDEDIDIVFQNCISFGLSLMSVVEQFTNVCPNRIILIPKLNEIHLMKPPIFTGDVDIHKLKQVIQKQHMFLPDKISLTQYVELFLSNKTLKSGSNVNSNLILAHKISDYFHNTYILSTNLAGHWILIIQLMKDSKGIFEKDWGEGYITDHMFINLKVFFNAYKTYLLCFHKGYGRAKLECDMNTSDLLCVLELIDSSYWKSMSKVFLEQKVIKYILSQDASLHRVKGCHSFKLWFLKRLNVAEFTVCPWVVNIDYHPTHMKAILTYIDLVRMGLINVDRIYIKNSHKFNDEFYTSNLFYINYNFSDNTHLLTKHIRIANSELESNYNKLYHPTPETLENILTNPVKSNGKKTLSDYCIGKNVDSIMLPSLSNKKLIKSSTMIRTNYSRQDLYNLFPTVVIDKIIDHSGNTAKSNQLYTTTSHQISLVHNSTSLYCMLPWHHINRFNFVFSSTGCKISIEYILKDLKIKDPNCIAFIGEGAGNLLLRTVVELHPDIRYIYRSLKDCNDHSLPIEFLRLYNGHINIDYGENLTIPATDATNNIHWSYLHIKFAEPISLFVCDAELPVTVNWSKIIIEWSKHVRKCKYCSSVNKCTLIVKYHAQDDIDFKLDNITILKTYVCLGSKLKGSEVYLVLTIGPANVFPVFNVVQNAKLILSRTKNFIMPKKADKESIDANIKSLIPFLCYPITKKGINTALSKLKSVVSGDILSYSIAGRNEVFSNKLINHKHMNILKWFNHVLNFRSTELNYNHLYMVESTYPHLSELLNSLTTNELKKLIKITGSLLYNFYNE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MZ151854
EMBL· GenBank· DDBJ
QYJ58005.1
EMBL· GenBank· DDBJ
Viral cRNA
MZ151855
EMBL· GenBank· DDBJ
QYJ58016.1
EMBL· GenBank· DDBJ
Viral cRNA

Similar Proteins

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