A0A8F4NUY3 · GKAC_PENCI

  • Protein
    Trans-enoyl reductase gkaC
  • Gene
    gkaC
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    3/5

Function

function

Trans-enoyl reductasee; part of the gene cluster that mediates the biosynthesis of GKK1032, fungal natural products containing a macrocyclic para-cyclophane connected to a decahydrofluorene ring system that show potent antitumor activities (PubMed:33834778).
Within the pathway, the PKS-NRPS gkaA, with the help of the trans-enoyl reductase gkaC, synthesize the polyketide-tyrosyl acyl thioester product which can be reductively off-loaded by the terminal reductase (R) domain in gkaA (PubMed:33834778).
The PKS module of gkaA acts in combination with the trans-acting enoyl reductase gkaC to produce a methylated polyketide attached to the ACP domain (PubMed:33834778).
In parallel, the adenylation (A) domain of the NRPS module activated L-tyrosine, which is then transferred to the ACP domain. The condensation (C) domain subsequently links this group to the polyketide chain, forming an enzyme-bound amide (PubMed:33834778).
The alpha/beta hydrolase gkaG is then required to catalyze the subsequent Knoevenagel condensation that affords the 3-pyrrolin-2-one ring, whereas the three proteins gkaB, gkadX and gkaZ then function synergistically to form the cyclophane (Probable)

Pathway

Mycotoxin biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site48-51NADP+ (UniProtKB | ChEBI)
Binding site172-175NADP+ (UniProtKB | ChEBI)
Binding site195-198NADP+ (UniProtKB | ChEBI)
Binding site213NADP+ (UniProtKB | ChEBI)
Binding site260-261NADP+ (UniProtKB | ChEBI)
Binding site351-352NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionnucleotide binding
Molecular Functionoxidoreductase activity, acting on NAD(P)H

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Trans-enoyl reductase gkaC
  • EC number
  • Alternative names
    • GKK1032 biosynthesis cluster protein C

Gene names

    • Name
      gkaC

Organism names

  • Taxonomic identifier
  • Strain
    • DSM 1997
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Penicillium

Accessions

  • Primary accession
    A0A8F4NUY3

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004584251-361Trans-enoyl reductase gkaC

Interaction

Subunit

Monomer.

Structure

3D structure databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain15-357Enoyl reductase (ER)

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    361
  • Mass (Da)
    39,162
  • Last updated
    2022-01-19 v1
  • Checksum
    EBB3B01CCA64CEB1
MDSLPTSQRAVVQSEDVGSFEISRGRPIPVPSSSQILIKVFAVALNHCDWKMPARVPCPGTVDGADYSGTIIALGDTAALTSGFKIGDRVAGAQMASQRRRPWVGAFTEYILEEADSAWLVPDSLSWEEAAAIGCAATSSVGMALWKSLNLPGTPQNPITEDGKYVLVYGGSSASGTFAIQLLRLSGYNVVTTCSPKNFGLVESYGAEKAFDYHSPTCGEDIRTYTGNTLEYALDIITEAKTIRHCYAAIGRGGGRYCGFELLPHDLIATMRRSVKAEWVNGLEMTGLEIDLPGGYYCKANPELHIWFADLIKQYSVLISQGKLRPHPIQINKGGLEKVIDGLGQMKRREISGKKMVYPLV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MW690135
EMBL· GenBank· DDBJ
QXF14612.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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