A0A8F1D6I8 · A0A8F1D6I8_9NIDO

  • Protein
    Replicase polyprotein 1ab
  • Gene
    ORF1ab
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    5/5

Function

function

Forms a primer, NSP9-pU, which is utilized by the polymerase for the initiation of RNA chains. Interacts with ribosome signal recognition particle RNA (SRP). Together with NSP8, suppress protein integration into the cell membrane, thereby disrupting host immune defenses.
RNA-directed RNA polymerase that catalyzes the transcription of viral genomic and subgenomic RNAs. Acts in complex with nsp7 and nsp8 to transcribe both the minus and positive strands of genomic RNA. The kinase-like NiRAN domain of NSP12 attaches one or more nucleotides to the amino terminus of NSP9, forming a covalent RNA-protein intermediate that serves as transcription/replication primer. Subgenomic RNAs (sgRNAs) are formed by discontinuous transcription: The polymerase has the ability to pause at transcription-regulating sequences (TRS) and jump to the leader TRS, resulting in a major deletion. This creates a series of subgenomic RNAs that are replicated, transcribed and translated. In addition, Nsp12 is a subunit of the viral RNA capping enzyme that catalyzes the RNA guanylyltransferase reaction for genomic and sub-genomic RNAs. Subsequently, the NiRAN domain transfers RNA to GDP, and forms the core cap structure GpppA-RNA.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site5686
Active site5688
Active site5787
Active site5863
Active site5868
Active site6340
Active site6355
Active site6396

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum-Golgi intermediate compartment
Cellular Componenthost cell membrane
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processsymbiont-mediated perturbation of host ubiquitin-like protein modification
Biological Processsymbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity
Biological Processviral genome replication
Biological Processviral protein processing
Biological Processviral translational frameshifting
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Replicase polyprotein 1ab
  • Alternative names
    • ORF1ab polyprotein

Gene names

    • Name
      ORF1ab

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • BtCoV/13585-35/M.dau/DK/2014
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Coronavirinae

Accessions

  • Primary accession
    A0A8F1D6I8

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1932-1952Helical
Transmembrane1999-2019Helical
Transmembrane2077-2094Helical
Transmembrane2101-2119Helical
Transmembrane2139-2158Helical
Transmembrane2502-2520Helical
Transmembrane2754-2778Helical
Transmembrane2790-2808Helical
Transmembrane2833-2861Helical
Transmembrane3284-3305Helical
Transmembrane3311-3330Helical
Transmembrane3335-3355Helical
Transmembrane3375-3393Helical
Transmembrane3405-3421Helical
Transmembrane3441-3464Helical
Transmembrane3471-3491Helical

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain2-109CoV Nsp1 globular
Domain112-361CoV Nsp2 N-terminal
Domain380-773CoV Nsp2 middle
Domain771-892CoV Nsp2 C-terminal
Domain893-988Ubiquitin-like
Domain1026-1281Peptidase C16
Domain1259-1426Macro
Domain1599-1654Ubiquitin-like
Domain1660-1924Peptidase C16
Domain2011-20753Ecto
Domain2149-2489CoV Nsp3 Y
Domain2876-2971Nsp4C
Domain2972-3273Peptidase C30
Domain3551-3633RdRp Nsp7 cofactor
Domain3634-3828RdRp Nsp8 cofactor
Domain3829-3936Nsp9 ssRNA-binding
Domain3937-4076ExoN/MTase coactivator
Domain4078-4327NiRAN
Domain4333-4431Nsp12 Interface
Domain4432-4999Nsp12 RNA-dependent RNA polymerase
Domain5000-5083CV ZBD
Domain5247-5608+RNA virus helicase C-terminal
Domain5668-5882ExoN
Domain5891-6112N7-MTase
Domain6115-6175Nsp15 N-terminal oligomerization
Domain6176-6293AV-Nsp11N/CoV-Nsp15M
Domain6310-6450NendoU
Domain6454-6750Nidovirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    6,754
  • Mass (Da)
    753,042
  • Last updated
    2022-01-19 v1
  • MD5 Checksum
    D304588DDD45BD8DD30FCECF08E66EB0

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MN535731
EMBL· GenBank· DDBJ
QWO79043.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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