A0A8E9YK14 · A0A8E9YK14_SALET
- Protein5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
- GenemtnN
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids232 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively. Also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine. Thus, is required for in vivo function of the radical SAM enzymes biotin synthase and lipoic acid synthase, that are inhibited by 5'-deoxyadenosine accumulation.
Catalytic activity
- 5'-deoxyadenosine + H2O = 5-deoxy-D-ribose + adenine
Pathway
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 12 | Proton acceptor | ||||
Sequence: E | ||||||
Binding site | 78 | substrate | ||||
Sequence: G | ||||||
Binding site | 152 | substrate | ||||
Sequence: I | ||||||
Binding site | 173-174 | substrate | ||||
Sequence: ME | ||||||
Active site | 197 | Proton donor | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | adenosylhomocysteine nucleosidase activity | |
Molecular Function | methylthioadenosine nucleosidase activity | |
Biological Process | L-methionine salvage from methylthioadenosine | |
Biological Process | L-methionine salvage from S-adenosylmethionine | |
Biological Process | purine deoxyribonucleoside catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
- EC number
- Short namesMTA/SAH nucleosidase ; MTAN
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella
Accessions
- Primary accessionA0A8E9YK14
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Homodimer.
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 2-226 | Nucleoside phosphorylase | ||||
Sequence: KIGIIGAMEEEVTLLRDKIDNRQTITLGGCEIYTGQLNGTEVALLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLASTLKVGDIVVSDEARYHDADVTAFGYEYGQLPGCPAGFKADDKLIAAAESCIRELNLNAVRGLIVSGDAFINGSVGLAKIRHNFPDAVAVEMEATAIAHVCYNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQSTLMVETLV |
Sequence similarities
Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length232
- Mass (Da)24,472
- Last updated2022-01-19 v1
- Checksum90FFC8E3DB5B9B26