A0A8E0FNS4 · A0A8E0FNS4_ECOLX

Function

function

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site118acetyl-CoA (UniProtKB | ChEBI)
Binding site125-126acetyl-CoA (UniProtKB | ChEBI)
Binding site274acetyl-CoA (UniProtKB | ChEBI)
Binding site311acetyl-CoA (UniProtKB | ChEBI)
Active site338Proton acceptor
Binding site338glyoxylate (UniProtKB | ChEBI)
Binding site427Mg2+ (UniProtKB | ChEBI)
Binding site427glyoxylate (UniProtKB | ChEBI)
Binding site452-455glyoxylate (UniProtKB | ChEBI)
Binding site455Mg2+ (UniProtKB | ChEBI)
Binding site536acetyl-CoA (UniProtKB | ChEBI)
Active site631Proton donor

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionmagnesium ion binding
Molecular Functionmalate synthase activity
Biological Processglyoxylate catabolic process
Biological Processglyoxylate cycle
Biological Processtricarboxylic acid cycle

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Malate synthase G
  • EC number

Gene names

    • Name
      glcB
    • ORF names
      EC970246_2876

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • 97.0246
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    A0A8E0FNS4

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue617Cysteine sulfenic acid (-SOH)
Modified residue688Cysteine sulfenic acid (-SOH)

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain16-77Malate synthase N-terminal
Domain158-233Malate synthase G alpha-beta insertion
Domain335-579Malate synthase TIM barrel
Domain591-695Malate synthase C-terminal

Sequence similarities

Belongs to the malate synthase family. GlcB subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    723
  • Mass (Da)
    80,438
  • Last updated
    2022-01-19 v1
  • Checksum
    38AAA4E9C92D463A
MSQTITQGRLRIDANFKRFVDEEVLPGVELDAAAFWHNVDEIVHDLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVETTGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDPQRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVGYRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVAAVDAEDKILLYRNLLGLMQGTLQEKMDKNGRQIVRKLNDDRQYTAADGSEISLHGRSLLFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPKMHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFINTGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGMWAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFNAEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIHNVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAGNFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AEZJ02000017
EMBL· GenBank· DDBJ
EIG93616.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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