A0A8D2FF15 · A0A8D2FF15_THEGE

Function

function

Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.

Miscellaneous

Ligation probably proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RTCB reacts with GTP to form a covalent RTCB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 manganese ions per subunit.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site119Mn2+ 1 (UniProtKB | ChEBI)
Binding site122Mn2+ 2 (UniProtKB | ChEBI)
Binding site122Mn2+ 1 (UniProtKB | ChEBI)
Binding site226-230GMP (UniProtKB | ChEBI)
Binding site227Mn2+ 1 (UniProtKB | ChEBI)
Binding site259Mn2+ 2 (UniProtKB | ChEBI)
Binding site353Mn2+ 2 (UniProtKB | ChEBI)
Binding site353-354GMP (UniProtKB | ChEBI)
Binding site402-405GMP (UniProtKB | ChEBI)
Binding site409GMP (UniProtKB | ChEBI)
Active site428GMP-histidine intermediate
Binding site428-431GMP (UniProtKB | ChEBI)
Binding site504GMP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentnucleus
Cellular ComponenttRNA-splicing ligase complex
Molecular FunctionGTP binding
Molecular Functionmetal ion binding
Molecular FunctionRNA ligase (ATP) activity
Molecular FunctionRNA ligase (GTP) activity
Biological ProcesstRNA splicing, via endonucleolytic cleavage and ligation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RNA-splicing ligase RtcB homolog
  • EC number
  • Alternative names
    • 3'-phosphate/5'-hydroxy nucleic acid ligase

Gene names

    • Name
      RTCB

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Cercopithecidae > Cercopithecinae > Theropithecus

Accessions

  • Primary accession
    A0A8D2FF15

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Catalytic component of the tRNA-splicing ligase complex.

Structure

3D structure databases

Family & Domains

Sequence similarities

Belongs to the RtcB family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    505
  • Mass (Da)
    55,200
  • Last updated
    2022-01-19 v1
  • Checksum
    173F91DE5D81CDCB
MSRNYNDELQFLEKISKNCWRIKKGFVPNMQVEGVFYVNDALEKLMFEELRNACRGGGVGGFLPAMKQIGNVAALPGIVHRSIGLPDVHSGYGFAIGNMAAFDMNDSEAVVSPGGVGFDINCGVRLLRTNLDESDVQPVKEQLAQAMFDHIPVGVGSKGVIPMNAKDLEEALEMGVDWSLREGYAWAEDKEHCEEYGRMLQADPNKVSARAKKRGLPQLGTLGAGNHYAEIQVVDEIFNEYAAKKMGIDHKGQVCVMIHSGSRGLGHQVATDALVAMEKAMKRDKIIVNDRQLACARIASPEGQDYLKGMAAAGNYAWVNRSSMTFLTRQAFAKVFNTTPDDLDLHVIYDVSHNIAKVEQHVVDGKERTLLVHRKGSTRAFPPHHPLIAVDYQLTGQPVLIGGTMGTCSYVLTGTEQGMTETFGTTCHGAGRALSRAKSRRNLDFQDVLDKLADMGIAIRVASPKLVMEEAPESYKNVTDVVNTCHDAGISKKAIKLRPIAVIKG

Keywords

Genome annotation databases

Similar Proteins

Disclaimer

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