A0A8D2F0I4 · A0A8D2F0I4_THEGE
- ProteinDouble-strand break repair protein
- GeneMRE11
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids708 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex 1 mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is 2 required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR. It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation, which is required for single-strand invasion and recombination.
Cofactor
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 129 | Proton donor | ||||
Sequence: H |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chromosome, telomeric region | |
Cellular Component | Mre11 complex | |
Cellular Component | site of double-strand break | |
Molecular Function | 3'-5'-DNA exonuclease activity | |
Molecular Function | manganese ion binding | |
Molecular Function | single-stranded DNA endodeoxyribonuclease activity | |
Biological Process | double-strand break repair via homologous recombination | |
Biological Process | double-strand break repair via nonhomologous end joining | |
Biological Process | meiotic DNA double-strand break formation | |
Biological Process | mitochondrial double-strand break repair via homologous recombination | |
Biological Process | mitotic G2 DNA damage checkpoint signaling | |
Biological Process | mitotic intra-S DNA damage checkpoint signaling | |
Biological Process | telomere maintenance |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameDouble-strand break repair protein
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Cercopithecidae > Cercopithecinae > Theropithecus
Accessions
- Primary accessionA0A8D2F0I4
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain, compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 294-461 | Mre11 DNA-binding | ||||
Sequence: MNMQKIPLHTVRQFFMEDIVLANHPDIFNPDNPKVTQAIQSFCLEKIEEMLENAERERLGNSRQPEKPLVRLRVDYSGGFEPFSVLRFSQKFVDRVANPKDIIHFFRHREQKEKTGEEINFGKLITKPSEGTTLRVEDLVKQYFQTAEKNVQLSLLTERGMGEAVQEF | ||||||
Compositional bias | 505-528 | Basic and acidic residues | ||||
Sequence: RESRQKNTNEEDDEVREAMTRARA | ||||||
Region | 505-540 | Disordered | ||||
Sequence: RESRQKNTNEEDDEVREAMTRARALRSQTEESASAF | ||||||
Region | 556-614 | Disordered | ||||
Sequence: NDSDDSISAATNKGRGRGRGRRGGRGQNSASRGGSQRGRADTGLETSTRSRNSKTAVSA | ||||||
Compositional bias | 583-614 | Polar residues | ||||
Sequence: NSASRGGSQRGRADTGLETSTRSRNSKTAVSA | ||||||
Region | 645-708 | Disordered | ||||
Sequence: EVDESDEEEDIFPTTSKTDQRWSSTSSSKIMSQSQVSKGVDFESSEDDDDDPFMNTSSLRRNRR | ||||||
Compositional bias | 660-684 | Polar residues | ||||
Sequence: SKTDQRWSSTSSSKIMSQSQVSKGV |
Sequence similarities
Belongs to the MRE11/RAD32 family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length708
- Mass (Da)80,561
- Last updated2022-01-19 v1
- Checksum33AB5F7DB043DBBD
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8D2EY50 | A0A8D2EY50_THEGE | MRE11 | 680 | ||
A0A8D2F172 | A0A8D2F172_THEGE | MRE11 | 648 | ||
A0A8D2F0C5 | A0A8D2F0C5_THEGE | MRE11 | 221 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 505-528 | Basic and acidic residues | ||||
Sequence: RESRQKNTNEEDDEVREAMTRARA | ||||||
Compositional bias | 583-614 | Polar residues | ||||
Sequence: NSASRGGSQRGRADTGLETSTRSRNSKTAVSA | ||||||
Compositional bias | 660-684 | Polar residues | ||||
Sequence: SKTDQRWSSTSSSKIMSQSQVSKGV |
Keywords
- Technical term