A0A8C2NC66 · A0A8C2NC66_CAPHI
- ProteinDNA repair nuclease/redox regulator APEX1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids272 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.
Catalytic activity
Cofactor
Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Probably binds two magnesium or manganese ions per subunit.
Features
Showing features for active site, binding site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 125 | ||||
Active site | 164 | Proton donor/acceptor | |||
Binding site | 164 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 166 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Site | 166 | Transition state stabilizer | |||
Site | 237 | Important for catalytic activity | |||
Binding site | 262 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Active site | 263 | Proton acceptor | |||
Binding site | 263 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Site | 263 | Interaction with DNA substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Cellular Component | nucleus | |
Molecular Function | DNA binding | |
Molecular Function | DNA-(apurinic or apyrimidinic site) endonuclease activity | |
Molecular Function | double-stranded DNA 3'-5' DNA exonuclease activity | |
Molecular Function | endonuclease activity | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoric diester hydrolase activity | |
Biological Process | base-excision repair | |
Biological Process | DNA recombination |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA repair nuclease/redox regulator APEX1
- EC number
- Short namesAPEN ; REF-1
- Alternative names
- Cleaved into 1 chains
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Caprinae > Capra
Accessions
- Primary accessionA0A8C2NC66
Proteomes
Subcellular Location
Structure
Sequence
- Sequence statusComplete
- Length272
- Mass (Da)30,621
- Last updated2022-01-19 v1
- Checksum44F8644250EC6F7A