A0A8C1IBD2 · A0A8C1IBD2_CYPCA

Function

function

Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.

Catalytic activity

  • Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
    EC:3.1.11.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Probably binds two magnesium or manganese ions per subunit.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site, site.

134150100150200250300
TypeIDPosition(s)Description
Binding site92Mg2+ 1 (UniProtKB | ChEBI)
Binding site120Mg2+ 1 (UniProtKB | ChEBI)
Active site195
Active site234Proton donor/acceptor
Binding site234Mg2+ 1 (UniProtKB | ChEBI)
Binding site236Mg2+ 1 (UniProtKB | ChEBI)
Site236Transition state stabilizer
Site306Important for catalytic activity
Binding site331Mg2+ 1 (UniProtKB | ChEBI)
Active site332Proton acceptor
Binding site332Mg2+ 1 (UniProtKB | ChEBI)
Site332Interaction with DNA substrate

GO annotations

AspectTerm
Cellular Componentmitochondrion
Cellular Componentnucleus
Molecular FunctionDNA binding
Molecular FunctionDNA-(apurinic or apyrimidinic site) endonuclease activity
Molecular Functiondouble-stranded DNA 3'-5' DNA exonuclease activity
Molecular Functionendonuclease activity
Molecular Functionmetal ion binding
Molecular Functionphosphoric diester hydrolase activity
Biological Processbase-excision repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    exodeoxyribonuclease III
  • EC number
  • Alternative names
    • APEX nuclease
    • Apurinic-apyrimidinic endonuclease 1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Cyprinidae > Cyprininae > Cyprinus

Accessions

  • Primary accession
    A0A8C1IBD2

Proteomes

Subcellular Location

Nucleus
Cytoplasm
Mitochondrion

Keywords

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-17
ChainPRO_503417480818-341exodeoxyribonuclease III

Family & Domains

Features

Showing features for compositional bias, region, domain.

TypeIDPosition(s)Description
Compositional bias35-81Basic and acidic residues
Region35-83Disordered
Domain90-332Endonuclease/exonuclease/phosphatase

Sequence similarities

Belongs to the DNA repair enzymes AP/ExoA family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    341
  • Mass (Da)
    38,394
  • Last updated
    2022-01-19 v1
  • Checksum
    8B40D780192E475E
MHFGLCSSRILCVRVCAVSFLRVCVLKTTVSMPKRAKKNEEAVEGETGNGAEPAKKEKKGKEPEAPILYEDPPDKMSSKDGRASNMKITSWNVDGLRAWVKKNGLDWVRKEDPDVLCLQETKCAEKALPSAITDMPEYPHKYWAGSEDKEGYSGVAMLCKTEPLNVTYGIGKEEHDKEGRVITAEFPSFFLVTAYVPNASRGLVRLDYRKTWDVDFRAYLSGLDQRKPLVLCGDLNVAHQEIDLKNPKGNRKNAGFTPEEREGFTKLLEAGFTDSFRELYPEQANAYTFWTYMMNARAKNVGWRLDYFLLSSALFPGLCDSKIRNTAMGSDHCPITLYLAV

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias35-81Basic and acidic residues

Keywords

Genome annotation databases

Similar Proteins

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