A0A8B9XVU1 · A0A8B9XVU1_BOSMU

Function

function

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.

Catalytic activity

  • (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + reduced [NADPH--hemoprotein reductase] + O2 = 21-hydroxy-(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+
    This reaction proceeds in the forward direction.
  • (5Z,8Z,11Z)-eicosatrienoate + reduced [NADPH--hemoprotein reductase] + O2 = 19-hydroxy-(5Z,8Z,11Z)-eicosatrienoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+
    This reaction proceeds in the forward direction.
  • (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + reduced [NADPH--hemoprotein reductase] + O2 = 19-hydroxy-(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+
    This reaction proceeds in the forward direction.
  • tetradecanoate + reduced [NADPH--hemoprotein reductase] + O2 = 13-hydroxytetradecanoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+
    This reaction proceeds in the forward direction.
  • an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H+
    This reaction proceeds in the forward direction.
    EC:1.14.14.1 (UniProtKB | ENZYME | Rhea)
  • an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H+
    This reaction proceeds in the forward direction.
  • dodecanoate + reduced [NADPH--hemoprotein reductase] + O2 = 11-hydroxydodecanoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+
    This reaction proceeds in the forward direction.

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Activity regulation

The omega-1 hydroxylase activity is stimulated by cytochrome b5.

Pathway

Lipid metabolism; fatty acid metabolism.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site435Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Cellular Componentmitochondrial inner membrane
Molecular Functionarachidonic acid epoxygenase activity
Molecular Functionaromatase activity
Molecular Functionheme binding
Molecular FunctionHsp70 protein binding
Molecular FunctionHsp90 protein binding
Molecular Functioniron ion binding
Biological Processepoxygenase P450 pathway
Biological Processxenobiotic metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

Gene names

    • Name
      CYP2E1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos

Accessions

  • Primary accession
    A0A8B9XVU1

Proteomes

Subcellular Location

Endoplasmic reticulum membrane
; Peripheral membrane protein
Microsome membrane
; Peripheral membrane protein
Mitochondrion inner membrane
; Peripheral membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane6-23Helical

Keywords

Interaction

Subunit

Interacts with chaperones HSP70 and HSP90; this interaction is required for initial targeting to mitochondria.

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    493
  • Mass (Da)
    56,572
  • Last updated
    2022-01-19 v1
  • Checksum
    F0FB3D719CB4E353
MAALGITVALLVWMATLLFISIWKHIYSSWKLPPGPFPLPIIGNLLQLDIKNIPKSFTRLAERYGPVFTLYLGSQRAVVVHGYKPVKEVLLDYKNEFSGRGENPGFQEHKNNGIIFNNGSTWRDTRRFSLTTLRDLGMGKQGNEQRIQREAHFLLEVLRKTQGQPFDPTFVVGFAPYNVISDILFHKRFDYKDQTSLRLMSLFNENFYLLSSPWIQLYNNFSDYLQYLPGSHKATENVSEVKYALERVKDHQKSLEPSCPRGFLDTMLIEMAKERHSVDPMYTLENIAVTVADLLFAGTETTSTTLRYGLLILMKYPEVEEKLHEEIDRVIGPSRIPAIKDRLDMPYLDAVVHEIQRFIDLIPSNLLHKATQDTVFRGYVIPKGTVIIPTLDSVLYDRQEFPEPEKFKPEHFLNENGKFKYSDHFKAFSAGKRVCVGEGLARMELFLLLAAILQHFNLKSLVDPKDIDLSPIAIGFGKIPPRYKLCLIPRSKV

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8B9Y182A0A8B9Y182_BOSMUCYP2E1509
A0A8B9XXY1A0A8B9XXY1_BOSMUCYP2E1495

Keywords

Genome annotation databases

Similar Proteins

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