A0A8B9XVU1 · A0A8B9XVU1_BOSMU
- ProteinCytochrome P450 2E1
- GeneCYP2E1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids493 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.
Catalytic activity
- (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + reduced [NADPH--hemoprotein reductase] + O2 = 21-hydroxy-(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+This reaction proceeds in the forward direction.
- (5Z,8Z,11Z)-eicosatrienoate + reduced [NADPH--hemoprotein reductase] + O2 = 19-hydroxy-(5Z,8Z,11Z)-eicosatrienoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+This reaction proceeds in the forward direction.
- (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + reduced [NADPH--hemoprotein reductase] + O2 = 19-hydroxy-(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+This reaction proceeds in the forward direction.
- tetradecanoate + reduced [NADPH--hemoprotein reductase] + O2 = 13-hydroxytetradecanoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+This reaction proceeds in the forward direction.
- an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H+This reaction proceeds in the forward direction.
- dodecanoate + reduced [NADPH--hemoprotein reductase] + O2 = 11-hydroxydodecanoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+This reaction proceeds in the forward direction.
Cofactor
Activity regulation
The omega-1 hydroxylase activity is stimulated by cytochrome b5.
Pathway
Lipid metabolism; fatty acid metabolism.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 435 | Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | mitochondrial inner membrane | |
Molecular Function | arachidonic acid epoxygenase activity | |
Molecular Function | aromatase activity | |
Molecular Function | heme binding | |
Molecular Function | Hsp70 protein binding | |
Molecular Function | Hsp90 protein binding | |
Molecular Function | iron ion binding | |
Biological Process | epoxygenase P450 pathway | |
Biological Process | xenobiotic metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytochrome P450 2E1
- EC number
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos
Accessions
- Primary accessionA0A8B9XVU1
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Peripheral membrane protein
Microsome membrane ; Peripheral membrane protein
Mitochondrion inner membrane ; Peripheral membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 6-23 | Helical | |||
Keywords
- Cellular component
Interaction
Subunit
Interacts with chaperones HSP70 and HSP90; this interaction is required for initial targeting to mitochondria.
Structure
Sequence
- Sequence statusComplete
- Length493
- Mass (Da)56,572
- Last updated2022-01-19 v1
- ChecksumF0FB3D719CB4E353
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8B9Y182 | A0A8B9Y182_BOSMU | CYP2E1 | 509 | ||
A0A8B9XXY1 | A0A8B9XXY1_BOSMU | CYP2E1 | 495 |
Keywords
- Technical term