A0A8B8UUE3 · A0A8B8UUE3_SACPA
- ProteinInosine triphosphate pyrophosphatase
- GeneHAM1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids197 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Pyrophosphatase that hydrolyzes the non-canonical purine (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP) and 5-bromodeoxyuridine 5'-triphosphate (BrdUTP) to their respective monophosphate derivatives. Xanthosine 5'-triphosphate (XTP) is also a potential substrate. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.
Catalytic activity
- H2O + ITP = diphosphate + H+ + IMP
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 1 divalent metal cation per subunit; can use either Mg2+ or Mn2+.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 10-15 | ITP (UniProtKB | ChEBI) | ||||
Sequence: TGNANK | ||||||
Binding site | 45 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 58 | ITP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 76 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 76-77 | ITP (UniProtKB | ChEBI) | ||||
Sequence: DT | ||||||
Binding site | 151-154 | ITP (UniProtKB | ChEBI) | ||||
Sequence: FGWD | ||||||
Binding site | 175 | ITP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 180-181 | ITP (UniProtKB | ChEBI) | ||||
Sequence: HR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | metal ion binding | |
Molecular Function | nucleoside triphosphate diphosphatase activity | |
Molecular Function | nucleotide binding | |
Biological Process | deoxyribonucleoside triphosphate catabolic process | |
Biological Process | nucleotide metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameInosine triphosphate pyrophosphatase
- EC number
- Short namesITPase ; Inosine triphosphatase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionA0A8B8UUE3
Organism-specific databases
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length197
- Mass (Da)22,011
- Last updated2022-01-19 v1
- Checksum83BD756B0D801939