A0A8B7FID6 · A0A8B7FID6_MICMU

Function

function

Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.

Catalytic activity

  • Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
    EC:3.1.11.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Probably binds two magnesium or manganese ions per subunit.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site, site.

TypeIDPosition(s)Description
Binding site68Mg2+ 1 (UniProtKB | ChEBI)
Binding site96Mg2+ 1 (UniProtKB | ChEBI)
Active site171
Active site210Proton donor/acceptor
Binding site210Mg2+ 2 (UniProtKB | ChEBI)
Binding site212Mg2+ 2 (UniProtKB | ChEBI)
Site212Transition state stabilizer
Site283Important for catalytic activity
Binding site308Mg2+ 1 (UniProtKB | ChEBI)
Active site309Proton acceptor
Binding site309Mg2+ 2 (UniProtKB | ChEBI)
Site309Interaction with DNA substrate

GO annotations

AspectTerm
Cellular Componentcentrosome
Cellular Componentmitochondrion
Cellular Componentnuclear speck
Cellular Componentnucleolus
Cellular Componentperinuclear region of cytoplasm
Molecular Functionchromatin DNA binding
Molecular Functionclass II DNA-(apurinic or apyrimidinic site) endonuclease activity
Molecular FunctionDNA-(abasic site) binding
Molecular Functiondouble-stranded DNA 3'-5' DNA exonuclease activity
Molecular Functiondouble-stranded telomeric DNA binding
Molecular Functionlyase activity
Molecular Functionmetal ion binding
Molecular Functionoxidoreductase activity
Molecular Functionphosphodiesterase activity, acting on 3'-phosphoglycolate-terminated DNA strands
Molecular Functionsite-specific endodeoxyribonuclease activity, specific for altered base
Molecular Functiontranscription coactivator activity
Biological Processcell redox homeostasis
Biological ProcessDNA catabolic process
Biological ProcessDNA recombination
Biological Processpositive regulation of gene expression via chromosomal CpG island demethylation
Biological Processpositive regulation of transcription by RNA polymerase II
Biological Processregulation of apoptotic process
Biological Processregulation of mRNA stability
Biological Processtelomere maintenance via base-excision repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA repair nuclease/redox regulator APEX1
  • EC number
  • Short names
    APEN
    ; REF-1
  • Alternative names
    • APEX nuclease
    • Apurinic-apyrimidinic endonuclease 1
    • Redox factor-1
  • Cleaved into 1 chains

Gene names

    • Name
      APEX1

Organism names

Accessions

  • Primary accession
    A0A8B7FID6

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for compositional bias, region, domain.

TypeIDPosition(s)Description
Compositional bias1-41Basic and acidic residues
Region1-59Disordered
Domain65-309Endonuclease/exonuclease/phosphatase

Sequence similarities

Belongs to the DNA repair enzymes AP/ExoA family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    318
  • Mass (Da)
    35,608
  • Last updated
    2022-01-19 v1
  • Checksum
    60072274B75BCC8E
MPKRGKKGAVAEDGEDPKTEPETKKSKTGAKKNEKEAAGEGPVLYEDPPDQKTSPSGKSATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPTELQELAGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGEEEHDQEGRVIVAEYDKFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTAYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8C5VP09A0A8C5VP09_MICMUAPEX114

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-41Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
ABDC03004434
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

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