A0A8B6LEQ1 · A0A8B6LEQ1_9BURK
- ProteinPhosphoenolpyruvate-protein phosphotransferase
- GeneptsI
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids603 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).
Catalytic activity
- L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-L-histidyl-[protein] + pyruvate
Cofactor
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 218 | Tele-phosphohistidine intermediate | ||||
Sequence: H | ||||||
Binding site | 325 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 366 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 466 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 489-490 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: ND | ||||||
Binding site | 490 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 500 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Active site | 537 | Proton donor | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | kinase activity | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoenolpyruvate-protein phosphotransferase activity | |
Biological Process | phosphoenolpyruvate-dependent sugar phosphotransferase system |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoenolpyruvate-protein phosphotransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Comamonadaceae > Variovorax
Accessions
- Primary accessionA0A8B6LEQ1
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 6-133 | Phosphotransferase system enzyme I N-terminal | ||||
Sequence: HGLPVARGIAIGRAVLVVSSRIDVAHYFIKPEQIETEIDRVRTARNAVSEELQKLQASVALMGPNDTPHELAALLDVHQMLLQDEVLTGGVKHWITERLYNAEWALTTQLEVIARQFDEMEDEYLRER | ||||||
Region | 157-176 | Disordered | ||||
Sequence: PTPPRRKRAAETEEGGDDPV | ||||||
Domain | 183-254 | PEP-utilising enzyme mobile | ||||
Sequence: APLVLIAHDLSPADMLQFKKSVFAGFVTDVGGRTSHTAIVARSMDIPAVVGARSASQLVRQDDWVIIDGDTG | ||||||
Domain | 281-575 | PEP-utilising enzyme C-terminal | ||||
Sequence: RLARLRHKPAVTLDGQRVDLLANIEMPEDTIGAVKAGAVGVGLFRSEFLFMGREAQRLTRLPDEEEQYQAYKRAVEGMQGMPVTIRTIDVGADKPLDGKSVRDDAHLNPALGLRAIRWSLADPAMFLTQLRAILRAAAHGEIHLLIPMLAHASEIRQTISLIDFARAELDNRGAVHGAVKLGAMIEIPAAALTLKTFLKHFDFLSIGTNDLIQYTLAIDRADESVAHLYDPAHPAVLRLVADTIAECRRQGKGVSVCGEMAGDIVFTRLLLGLGLRSFSMHPSQILAVKQEVLRA |
Sequence similarities
Belongs to the PEP-utilizing enzyme family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length603
- Mass (Da)66,088
- Last updated2021-09-29 v1
- Checksum7144D9FAD949F5BA