A0A8B6LEM0 · A0A8B6LEM0_9BURK

  • Protein
    Phosphoenolpyruvate carboxykinase [GTP]
  • Gene
    pckG
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 Mn2+ ion per subunit.

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site83substrate
Binding site222-224substrate
Binding site231Mn2+ (UniProtKB | ChEBI)
Binding site251Mn2+ (UniProtKB | ChEBI)
Binding site273substrate
Binding site274-279GTP (UniProtKB | ChEBI)
Active site275
Binding site300Mn2+ (UniProtKB | ChEBI)
Binding site393-395substrate
Binding site395GTP (UniProtKB | ChEBI)
Binding site426GTP (UniProtKB | ChEBI)
Binding site523-526GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionGTP binding
Molecular Functionkinase activity
Molecular Functionmanganese ion binding
Molecular Functionphosphoenolpyruvate carboxykinase (GTP) activity
Biological Processcellular response to glucose stimulus
Biological Processgluconeogenesis
Biological Processglycerol biosynthetic process from pyruvate
Biological Processpropionate catabolic process
Biological Processresponse to lipid
Biological Processresponse to starvation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphoenolpyruvate carboxykinase [GTP]
  • EC number
  • Short names
    PEP carboxykinase
    ; PEPCK
  • Alternative names
    • GTP-dependent phosphoenolpyruvate carboxykinase
      (GTP-PEPCK
      )

Gene names

    • Name
      pckG
    • ORF names
      SRS16CHR_00165

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • SRS16
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Comamonadaceae > Variovorax

Accessions

  • Primary accession
    A0A8B6LEM0

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain24-243Phosphoenolpyruvate carboxykinase GTP-utilising N-terminal
Domain247-608Phosphoenolpyruvate carboxykinase C-terminal P-loop

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    615
  • Mass (Da)
    67,400
  • Last updated
    2021-09-29 v1
  • Checksum
    F36B39C81A9D7326
MNAPVKGLAVQAPDYVKNAKLIAWVADMAALCKPESIHWCDGSSEEYERLCQQMVEAGTLKKLNPAKRPNSYLASSDPGDVARVEDRTFICAPRKEDAGPTNNWMAPADMRATLQPLFDGSMKGRTMYVVPFSMGPLGSPIAHIGIELSDSPYVAVNMKIMTRMGKAVYDVLGADGDFVPCVHTVGAPLEPGQKDVSWPCNKTKYIVHYPETREIWSYGSGYGGNALLGKKCFALRIASNMGRDEGWLAEHMLILGVTNPAGRKYHVAAAFPSACGKTNFAMLIPPAGFEGWKVTTIGDDIAWIKPGADGKLHAINPEAGYFGVAPGTNTLTNPNCMASLDRDVIFTNVALTDDGDVWWEGMSEPPAHAIDWQGKDWTPAIARETGAKAAHPNARFTVAATNNPALDDAWDDPKGVAIDAFIFGGRRSTTVPLVTEARDWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFAGYNMSDYFQHWLDLGKKLEASDARLPKIYTTNWFRKGEDGKFVWPGYGENMRVLKWMIDRVEQKAEGVEHVFGVSPRYEDLDWTGLDFSARQFDTVTSIDKAAWHAELKLHAELFQQLAHHLPKELPAIKAAIELRLAA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LR594666
EMBL· GenBank· DDBJ
VTU12926.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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