A0A8A4JKT9 · A0A8A4JKT9_CHIKV

Function

function

Inactive precursor of the viral replicase, which is activated by cleavages carried out by the viral protease nsP2.

Catalytic activity

  • 4-O-(ADP-D-ribosyl)-L-aspartyl-[protein] + H2O = ADP-D-ribose + H+ + L-aspartyl-[protein]
    This reaction proceeds in the forward direction.
  • 5-O-(ADP-D-ribosyl)-L-glutamyl-[protein] + H2O = ADP-D-ribose + H+ + L-glutamyl-[protein]
    This reaction proceeds in the forward direction.
  • ADP-alpha-D-ribose 1''-phosphate + H2O = ADP-D-ribose + phosphate
    This reaction proceeds in the forward direction.
    EC:3.1.3.84 (UniProtKB | ENZYME | Rhea)
  • ATP + H2O = ADP + H+ + phosphate
    EC:3.6.4.13 (UniProtKB | ENZYME | Rhea)
  • ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide
    EC:2.7.7.19 (UniProtKB | ENZYME | Rhea)
  • GTP + S-adenosyl-L-methionine = N7-methyl-GTP + S-adenosyl-L-homocysteine
  • L-histidyl-[protein] + N7-methyl-GTP = diphosphate + N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein]
    This reaction proceeds in the forward direction.
  • a 5'-end diphospho-(purine-ribonucleoside) in mRNA + H+ + N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein] = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(purine-ribonucleoside) in mRNA + L-histidyl-[protein]
  • a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + H+ + phosphate
    This reaction proceeds in the forward direction.
    EC:3.6.1.74 (UniProtKB | ENZYME | Rhea)
  • a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + H+ + phosphate
    EC:3.6.1.15 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site1003For cysteine protease nsP2 activity
Active site1073For cysteine protease nsP2 activity

GO annotations

AspectTerm
Cellular Componentcytoplasmic vesicle membrane
Cellular Componenthost cell cytoplasmic vesicle membrane
Cellular Componenthost cell filopodium
Cellular Componenthost cell nucleus
Cellular Componenthost cell plasma membrane
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular Functioncysteine-type peptidase activity
Molecular FunctionGTP binding
Molecular Functionhelicase activity
Molecular Functionmetal ion binding
Molecular FunctionmRNA methyltransferase activity
Molecular FunctionRNA binding
Molecular FunctionRNA-dependent RNA polymerase activity
Biological Process7-methylguanosine mRNA capping
Biological ProcessDNA-templated transcription
Biological Processmethylation
Biological ProcessmRNA modification
Biological Processproteolysis
Biological Processsymbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
Biological Processviral RNA genome replication

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Polyprotein P1234
  • Alternative names
    • Non-structural polyprotein

Organism names

Accessions

  • Primary accession
    A0A8A4JKT9

Subcellular Location

Cell membrane
; Lipid-anchor
Cytoplasmic vesicle membrane
; Lipid-anchor
Cytoplasmic vesicle membrane
; Peripheral membrane protein
Host cell membrane
; Lipid-anchor
Host cytoplasmic vesicle membrane
; Lipid-anchor
Host cytoplasmic vesicle membrane
; Peripheral membrane protein
Host nucleus
Membrane
; Lipid-anchor
Membrane
; Peripheral membrane protein

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain18-249Alphavirus-like MT
Region475-495Disordered
Compositional bias478-495Basic and acidic residues
Domain680-981+RNA virus helicase C-terminal
Domain994-1317Peptidase C9
Domain1314-1483Macro
Region1642-1665Disordered
Domain2218-2333RdRp catalytic

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,464
  • Mass (Da)
    274,937
  • Last updated
    2021-09-29 v1
  • Checksum
    98FFA604D5AB79FD
DSAFLKALQRAYPMFEVEPRQVTPNDHANARAFSHLAIKLIEQEIDPDSTILDIGSAPARRMMSDRKYHCVCPMRSAEDPERLANYARKLASAAGKVLDRNISGKIGDLQEVMAVPDTETPTFCLHTDVSCRQRADVAIYQDVYAVHAPTSLYHQAIKGFRLAYWVGFDTTPFMYNAMAGAYPSYSTNWADEQVLKAKNIGLCSTDLTEGRRGKLSIMRGKKLKPCDRVLFSVGSTLYSESRKLLKSWHLPSVFHLKGKLSFTCRCDTVVSCEGYVVKRITMSPGLYGKTTGYAVTHHADGFLMCKTTDTVDGERVSFSVCTYVPATICDQMTGILATEVTPEDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPVVAQAFSKWAKECRKDMEDEKLLGVRERTLTCCCLWAFKKQKTHTVYKRPDTQSIQKVQAEFDSFVVPSLWSSGLSIPLRTRIKWLLSKVPKTDLIPYSGDAQEARDAEKEAEEEREAELTREALPPLQAAQEDVQVEIDVEQLEDRAGAGIIETPRGAIKVTAQPTDHVVGEYLVLSPQTVLRSQKLSLIHALAEQVKTCTHSGRAGRYAVEAYDGRVLVPSGYAISPEDFQSLSESATMVYNEREFVNRKLHHIAMHGPALNTDEESYELVRAERTEHEYVYDVDQRRCCKKEEAAGLVLVGDLTNPPYHEFAYEGLKIRPACPYKIAVIGVFGVPGSGKSAIIKNLVTRQDLVTSGKKENCQEITTDVMRQRGLEISARTVDSLLLNGCNRPVDVLYVDEAFACHSGTLLALIALVRPRQKVVLCGDPKQCGFFNMMQMKVNYNHNICTQVYHKSISRRCTLPVTAIVSSLHYEGKMRTTNEYNKPIVVDTTGSTKPDAGDLVLTCFRGWVKQLQIDYRGYEVMTAAASQGLTRKGVYAVRQKVNENPLYASTSEHVNVLLTRTEGKLVWKTLSGDPWIKTLQNPPKGNFKATIKEWEVEHASIMAGICSHQLTFDTFQNKANVCWAKSLVPILETAGIKLNDRQWSQIIQAFKEDKAYSPEVALNEICTRMYGVDLDSGLFSKPLVSVYYSDNHWDNRPGGKMFGFNPEAASILERKYPFTKGKWNINKQICVTTRRIEDFNPTTNIIPANRRLPHSLVAEHRPVKGERMEWLVNKINGHHVLLVSGYNLVLPTKRVTWVAPLGVRGADYTYNLELGLPATLGRYDLVVINIHTPFRIHHYQQCVDHAMKLQMLGGDSLRLLKPGGSLLIRAYGYADRTSERVICVLGRKFRSSRALKPPCVTSNTEMFFLFSNFDNGRRNFTTHVMNNQLNAAFVGQATRAGCAPSYRVKRMDIAKNDEECVVNAANPRGLPGDGVCKAVYKKWPESFKNSATPVGTAKTVMCGTYPVIHAVGPNFSNYLESEGDRELAAAYREVAKEVTRLGVNSVAIPLLSTGVYSGGKDRLTQSLNHLFTAMDSTDADVVIYCRDKEWEKKISEAIQMRTQVELLDEHISIDCDIVRVHPDSSLAGRKGYSTTEGALYSYLEGTRFHQTAVDMAEIYTMWPKQTEANEQVCLYALGESIESIRQKCPVDDADASSPPKTVPCLCRYAMTPERVTRLRMNHVTSIIVCSSFPLPKYKIEGVQKVKCSKVMLFDHNVPSRVSPREYRSSPEAAQEASTTTSLTHSQFDLSVDGEILPVPSDLDADAPALEPALDDRATHTLPSTTGNLAAVSDWVMSTVPVAPPRRRRGRNLTVTCDEREGNITPMASVRFFRAELRPVVQETAETRDTAMSLQAPPSTATELNHPPISFGAPSETFPITFGDFNEGEIESLSSELLTFGDFLPGEVDDLTDSDWSTCSDTDDELRLDRAGGYIFSSDTGPGHLQQKSVRQSVLPVNTLEEVHEEKCYPPKLDEVKEQLLLKKLQESASMANRSRYQSRKVENMKATIIQRLKRGCRLYLMSETSKVPTYRTTYPAPVYSPPINVRLSNPESXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGSESCLDRATFNPSKLRSYPKQHAYHAPSIRSAVPSPFQNTLQNVLAAATKRNCNVTQMRELPTLDSAVFNVECFKKFACNQEYWEEFAASPIRITTENLTTYVTKLKGPKAAALFAKTHNLLPLQEVPMDRFTVDMKRDVKVTPGTKHTEERPKVQVIQAAEPLATAYLCGIHRELVRRLNAVLLPNVHTLFDMSAEDFDAIIAAHFKPGDTVLETDIASFDKSQDDSLALTALMLLEDLGVDHSLLDLIEAAFGEISSCHLPTGTRFKFGAMMKSGMFLTLFVNTLLNITIASRVLEDRLTKSACAAFIGDDNIIHGVVSDELMADRCATWMNMEVKIIDAVVSLKAPYFCGGFILHDTVTGTACRVADPLKRLFKLGKPLAAGDEQDEDRRRALADEVIRWQRTGLIDELEKAVYSRYEVQGISVVVMSMATFASSRSNFEKLRGPVITLYGGPK

Features

Showing features for non-terminal residue, compositional bias.

TypeIDPosition(s)Description
Non-terminal residue1
Compositional bias478-495Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MW260519
EMBL· GenBank· DDBJ
QTC35361.1
EMBL· GenBank· DDBJ
Genomic RNA

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