A0A8A4JKT9 · A0A8A4JKT9_CHIKV
- ProteinPolyprotein P1234
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids2464 (go to sequence)
- Protein existencePredicted
- Annotation score5/5
Function
function
Inactive precursor of the viral replicase, which is activated by cleavages carried out by the viral protease nsP2.
Catalytic activity
- 4-O-(ADP-D-ribosyl)-L-aspartyl-[protein] + H2O = ADP-D-ribose + H+ + L-aspartyl-[protein]This reaction proceeds in the forward direction.
- 5-O-(ADP-D-ribosyl)-L-glutamyl-[protein] + H2O = ADP-D-ribose + H+ + L-glutamyl-[protein]This reaction proceeds in the forward direction.
- GTP + S-adenosyl-L-methionine = N7-methyl-GTP + S-adenosyl-L-homocysteine
- L-histidyl-[protein] + N7-methyl-GTP = diphosphate + N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein]This reaction proceeds in the forward direction.
- a 5'-end diphospho-(purine-ribonucleoside) in mRNA + H+ + N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein] = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(purine-ribonucleoside) in mRNA + L-histidyl-[protein]
Cofactor
Protein has several cofactor binding sites:
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 1003 | For cysteine protease nsP2 activity | ||||
Sequence: C | ||||||
Active site | 1073 | For cysteine protease nsP2 activity | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasmic vesicle membrane | |
Cellular Component | host cell cytoplasmic vesicle membrane | |
Cellular Component | host cell filopodium | |
Cellular Component | host cell nucleus | |
Cellular Component | host cell plasma membrane | |
Cellular Component | plasma membrane | |
Molecular Function | ATP binding | |
Molecular Function | cysteine-type peptidase activity | |
Molecular Function | GTP binding | |
Molecular Function | helicase activity | |
Molecular Function | metal ion binding | |
Molecular Function | mRNA methyltransferase activity | |
Molecular Function | RNA binding | |
Molecular Function | RNA-dependent RNA polymerase activity | |
Biological Process | 7-methylguanosine mRNA capping | |
Biological Process | DNA-templated transcription | |
Biological Process | methylation | |
Biological Process | mRNA modification | |
Biological Process | proteolysis | |
Biological Process | symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity | |
Biological Process | viral RNA genome replication |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended namePolyprotein P1234
- Alternative names
Organism names
- Organism
- Strain
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Kitrinoviricota > Alsuviricetes > Martellivirales > Togaviridae > Alphavirus
- Virus hosts
Accessions
- Primary accessionA0A8A4JKT9
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Lipid-anchor
Cytoplasmic vesicle membrane ; Lipid-anchor
Cytoplasmic vesicle membrane ; Peripheral membrane protein
Host cell membrane ; Lipid-anchor
Host cytoplasmic vesicle membrane ; Lipid-anchor
Host cytoplasmic vesicle membrane ; Peripheral membrane protein
Membrane ; Lipid-anchor
Membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 18-249 | Alphavirus-like MT | ||||
Sequence: EPRQVTPNDHANARAFSHLAIKLIEQEIDPDSTILDIGSAPARRMMSDRKYHCVCPMRSAEDPERLANYARKLASAAGKVLDRNISGKIGDLQEVMAVPDTETPTFCLHTDVSCRQRADVAIYQDVYAVHAPTSLYHQAIKGFRLAYWVGFDTTPFMYNAMAGAYPSYSTNWADEQVLKAKNIGLCSTDLTEGRRGKLSIMRGKKLKPCDRVLFSVGSTLYSESRKLLKSWH | ||||||
Region | 475-495 | Disordered | ||||
Sequence: GDAQEARDAEKEAEEEREAEL | ||||||
Compositional bias | 478-495 | Basic and acidic residues | ||||
Sequence: QEARDAEKEAEEEREAEL | ||||||
Domain | 680-981 | +RNA virus helicase C-terminal | ||||
Sequence: DLTNPPYHEFAYEGLKIRPACPYKIAVIGVFGVPGSGKSAIIKNLVTRQDLVTSGKKENCQEITTDVMRQRGLEISARTVDSLLLNGCNRPVDVLYVDEAFACHSGTLLALIALVRPRQKVVLCGDPKQCGFFNMMQMKVNYNHNICTQVYHKSISRRCTLPVTAIVSSLHYEGKMRTTNEYNKPIVVDTTGSTKPDAGDLVLTCFRGWVKQLQIDYRGYEVMTAAASQGLTRKGVYAVRQKVNENPLYASTSEHVNVLLTRTEGKLVWKTLSGDPWIKTLQNPPKGNFKATIKEWEVEHAS | ||||||
Domain | 994-1317 | Peptidase C9 | ||||
Sequence: DTFQNKANVCWAKSLVPILETAGIKLNDRQWSQIIQAFKEDKAYSPEVALNEICTRMYGVDLDSGLFSKPLVSVYYSDNHWDNRPGGKMFGFNPEAASILERKYPFTKGKWNINKQICVTTRRIEDFNPTTNIIPANRRLPHSLVAEHRPVKGERMEWLVNKINGHHVLLVSGYNLVLPTKRVTWVAPLGVRGADYTYNLELGLPATLGRYDLVVINIHTPFRIHHYQQCVDHAMKLQMLGGDSLRLLKPGGSLLIRAYGYADRTSERVICVLGRKFRSSRALKPPCVTSNTEMFFLFSNFDNGRRNFTTHVMNNQLNAAFVGQ | ||||||
Domain | 1314-1483 | Macro | ||||
Sequence: FVGQATRAGCAPSYRVKRMDIAKNDEECVVNAANPRGLPGDGVCKAVYKKWPESFKNSATPVGTAKTVMCGTYPVIHAVGPNFSNYLESEGDRELAAAYREVAKEVTRLGVNSVAIPLLSTGVYSGGKDRLTQSLNHLFTAMDSTDADVVIYCRDKEWEKKISEAIQMRT | ||||||
Region | 1642-1665 | Disordered | ||||
Sequence: VSPREYRSSPEAAQEASTTTSLTH | ||||||
Domain | 2218-2333 | RdRp catalytic | ||||
Sequence: DTVLETDIASFDKSQDDSLALTALMLLEDLGVDHSLLDLIEAAFGEISSCHLPTGTRFKFGAMMKSGMFLTLFVNTLLNITIASRVLEDRLTKSACAAFIGDDNIIHGVVSDELMA |
Family and domain databases
Sequence
- Sequence statusComplete
- Length2,464
- Mass (Da)274,937
- Last updated2021-09-29 v1
- Checksum98FFA604D5AB79FD
Features
Showing features for non-terminal residue, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: D | ||||||
Compositional bias | 478-495 | Basic and acidic residues | ||||
Sequence: QEARDAEKEAEEEREAEL |
Keywords
- Coding sequence diversity