A0A8A0R1W6 · A0A8A0R1W6_TUPCH

Function

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate = (8S)-hydroxy-(11S,12S)-epoxy-(5Z,9E,14Z)-eicosatrienoate
    This reaction proceeds in the forward direction.
  • (14R,15S)-epoxy-(5Z,8Z,11Z)-eicosatrienoate + O2 = (12S)-hydroperoxy-(14R,15S)-epoxy-(5Z,8Z,10E)-eicosatrienoate
    This reaction proceeds in the forward direction.
  • (14R,15S)-epoxy-(5Z,8Z,11Z)-eicosatrienoate + O2 = (5S)-hydroperoxy-(14R,15S)-epoxy-(6E,8Z,11Z)-eicosatrienoate
    This reaction proceeds in the forward direction.
  • (14S,15R)-epoxy-(5Z,8Z,11Z)-eicosatrienoate + O2 = (12S)-hydroperoxy-(14S,15R)-epoxy-(5Z,8Z,10E)-eicosatrienoate
    This reaction proceeds in the forward direction.
  • (14S,15R)-epoxy-(5Z,8Z,11Z)-eicosatrienoate + O2 = (8S)-hydroperoxy-(14S,15R)-epoxy-(5Z,9E,11Z)-eicosatrienoate
    This reaction proceeds in the forward direction.
  • (15R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate = 15-oxo-(5Z,8Z,11Z,13E)-eicosatetraenoate + H2O
    This reaction proceeds in the forward direction.
  • (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate = (14S,15S)-epoxy-(5Z,8Z,10E,12E)-eicosatetraenoate + H2O
    This reaction proceeds in the forward direction.
  • (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate + O2 = 14-hydroperoxy-(4Z,7Z,10Z,12E,16Z)-docosapentaenoate
    This reaction proceeds in the forward direction.
  • (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (11S)-hydroperoxy-(4Z,7Z,9E,13Z,16Z,19Z)-docosahexaenoate
    This reaction proceeds in the forward direction.
  • (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (14S)-hydroperoxy-(4Z,7Z,10Z,12E,16Z,19Z)-docosahexaenoate
    This reaction proceeds in the forward direction.
  • (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (17S)-hydroperoxy-(4Z,7Z,10Z,13Z,15E,19Z)-docosahexaenoate
    This reaction proceeds in the forward direction.
  • (5Z,8Z,11Z,14Z)-eicosatetraenoate + 2 O2 = (14R,15S)-dihydroperoxy-(5Z,8Z,10E,12E)-eicosatetraenoate
    This reaction proceeds in the forward direction.
  • (5Z,8Z,11Z,14Z)-eicosatetraenoate + 2 O2 = (8S,15S)-dihydroperoxy-(5Z,9E,11Z,13E)-eicosatetraenoate
    This reaction proceeds in the forward direction.
  • (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate
    This reaction proceeds in the forward direction.
    EC:1.13.11.31 (UniProtKB | ENZYME | Rhea)
  • (7S)-hydroperoxy-(4Z,8E,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (7S,14S)-dihydroperoxy-(4Z,8E,10Z,12E,16Z,19Z)-docosahexaenoate
    This reaction proceeds in the forward direction.
  • (7S)-hydroperoxy-(4Z,8E,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (7S,17S)-dihydroperoxy-(4Z,8E,10Z,13Z,15E,19Z)-docosahexaenoate
    This reaction proceeds in the forward direction.
  • (7Z,10Z,13Z,16Z,19Z)-docosapentaenoate + O2 = 14-hydroperoxy-(7Z,10Z,12E,16Z,19Z)-docosapentaenoate
    This reaction proceeds in the forward direction.
  • (9Z,12Z)-octadecadienoate + O2 = (13S)-hydroperoxy-(9Z,11E)-octadecadienoate
    This reaction proceeds in the forward direction.
    EC:1.13.11.12 (UniProtKB | ENZYME | Rhea)
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-alanine + O2 = N-(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-alanine
    This reaction proceeds in the forward direction.
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-alanine + O2 = N-(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-alanine
    This reaction proceeds in the forward direction.
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-gamma-aminobutanoate + O2 = N-(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-gamma-aminobutanoate
    This reaction proceeds in the forward direction.
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-gamma-aminobutanoate + O2 = N-(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-gamma-aminobutanoate
    This reaction proceeds in the forward direction.
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine + O2 = N-(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-glycine
    This reaction proceeds in the forward direction.
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine + O2 = N-(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-glycine
    This reaction proceeds in the forward direction.
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-taurine + O2 = N-(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-taurine
    This reaction proceeds in the forward direction.
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-taurine + O2 = N-(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-taurine
    This reaction proceeds in the forward direction.

Cofactor

Fe cation (UniProtKB | Rhea| CHEBI:24875 )

Note: Binds 1 Fe cation per subunit.

Pathway

Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.

Features

Showing features for binding site, site.

Type
IDPosition(s)Description
Binding site17Ca2+ 1 (UniProtKB | ChEBI)
Binding site74Ca2+ 1 (UniProtKB | ChEBI)
Binding site75Ca2+ 1 (UniProtKB | ChEBI)
Site100Essential for stabilizing binding to COTL1
Binding site361Fe cation (UniProtKB | ChEBI); catalytic
Binding site366Fe cation (UniProtKB | ChEBI); catalytic
Binding site541Fe cation (UniProtKB | ChEBI); catalytic
Binding site663Fe cation (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentlipid droplet
Cellular Componentplasma membrane
Molecular Functionarachidonate 12(S)-lipoxygenase activity
Molecular Functionarachidonate 15-lipoxygenase activity
Molecular Functioniron ion binding
Molecular Functionlipid binding
Biological Processarachidonic acid metabolic process
Biological Processhepoxilin biosynthetic process
Biological Processlinoleic acid metabolic process
Biological Processlipid oxidation
Biological Processlipoxygenase pathway

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Polyunsaturated fatty acid lipoxygenase ALOX15
  • EC number
  • Alternative names
    • 12/15-lipoxygenase
    • Arachidonate 12-lipoxygenase, leukocyte-type
    • Arachidonate 15-lipoxygenase
    • Arachidonate omega-6 lipoxygenase
    • Hepoxilin A3 synthase Alox15
    • Linoleate 13S-lipoxygenase

Gene names

    • Name
      ALOX15a

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Scandentia > Tupaiidae > Tupaia

Accessions

  • Primary accession
    A0A8A0R1W6

Subcellular Location

Cell membrane
; Peripheral membrane protein
Cytoplasm, cytosol
Lipid droplet

Keywords

Interaction

Subunit

Interacts with PEBP1; in response to IL13/interleukin-13, prevents the interaction of PEBP1 with RAF1 to activate the ERK signaling cascade.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain2-115PLAT
Domain115-663Lipoxygenase

Sequence similarities

Belongs to the lipoxygenase family.

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    663
  • Mass (Da)
    75,206
  • Last updated
    2021-09-29 v1
  • Checksum
    C700622C0C3A424C
MVLYRIRVSTGSSCYAGSKNQVHLSLVGQHGEAALGWRLRPARGKVEEFQVDVQEYLGPLLFVKLRKWHLLQDDAWFCNWVSVQGPGASGDEVRFPFYRWVEGKDILSLPEATGRTVVDDPQGLFRRHREEELEDRKKVYRWGNWKDGLILNMAGPGLNDLPVDERFLEDKRIDFEASLAKGLAELAIKDSLNILANWNNVDDFNRIFWCGPSKLAVQVRDSWKEDALFGYQFLNGANPMLLRRSCHLPDRLVFPPGMEELRAQLENELRAGTLFEADYSLLDGIKANVILCRQQYLAAPLVMLKLQSDGKLLPMVIQLQLPQQGSPPPTLFLPTDPELTWLLAKCWVRSSEFQIHELQYHLLRGHLMAEVIAVATMRCLPSVHPIFKLIVPHLRYTMEINVRARNGLVSDYGVFDQVVSTGGGGHVEFLKRAKDVLTYRSLCPPDDLADRGLLGVQSSYYGQDALRLWEILYGYVEGIVKIHYKSDETVKSDLELQSWCREITEIGLLGAEDRGFPKSLQSLDQLCKFATMCIFTCTGQHSSNHLGQLDWYAWVPNAPCTMRIPPPTTKDVTMETVMASLPNVHQASLQMSITWQLGRRQPIMVALGQHEEEYFSDPASKAVLKTFREKLAAMDKDVDARNATLAMPYEYLKPSLVENSVAI

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue663

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MW690658
EMBL· GenBank· DDBJ
QSQ01680.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

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